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Osteopontin Rabbit Polyclonal Antibody

RMB: 900 特惠 1500

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Catalog# ER1802-16

Osteopontin Rabbit Polyclonal Antibody

Application

  • IF-Cell

  • IHC-P

  • ELISA

Reactivity

  • Human

  • Mouse

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Osteopontin Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within mouse Osteopontin 17-100.

种属反应性

Human, Mouse

验证应用

IF-Cell, IHC-P, ELISA

分子量

Predicted band size: 35 kDa

阳性对照

HepG2, MCF-7, human liver cancer tissue, human kidney tissue, mouse kidney tissue.

偶联

unconjugated

RRID

AB_3069113

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • ELISA

  • 1:5,000-1:10,000

发表文章中的种属

Mouse 查看 3 篇文献如下
Human 查看 3 篇文献如下

靶点

功能

Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.

背景文献

1. Young MF et al. cDNA cloning, mRNA distribution and heterogeneity, chromosomal location, and RFLP analysis of human osteopontin (OPN). Genomics 7(1): 491-502 (1990).

2. Barros NM et al. Proteolytic processing of osteopontin by PHEX and accumulation of osteopontin fragments in Hyp mouse bone, the murine model of X-linked hypophosphatemia. Journal of Bone and Mineral Research 28(3): 688-99 (2013).

序列相似性

Belongs to the osteopontin family.

组织特异性

Bone. Found in plasma.

翻译后修饰

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.; O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.

亚细胞定位

Secreted.

UNIPROT

SwissProt: P10451 Human

SwissProt: P10923 Mouse

别名

BNSP antibody

Bone sialoprotein 1 antibody

BSP I antibody

BSPI antibody

Early T lymphocyte activation 1 antibody

ETA 1 antibody

ETA1 antibody

MGC110940 antibody

Nephropontin antibody

OPN antibody

展开

图片

  • ICC staining Osteopontin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Osteopontin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Osteopontin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Osteopontin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of RAW264.7 cells treated with LPS (100 ng/ml 6h) add BFA (300 ng/ml 3h) labeling Osteopontin with Rabbit anti-Osteopontin antibody (<a href="/products/ER1802-16" style="font-weight: bold;text-decoration: underline;">ER1802-16</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Osteopontin antibody (<a href="/products/ER1802-16" style="font-weight: bold;text-decoration: underline;">ER1802-16</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of RAW264.7 cells treated with LPS (100 ng/ml 6h) add BFA (300 ng/ml 3h) labeling Osteopontin with Rabbit anti-Osteopontin antibody (ER1802-16) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Osteopontin antibody (ER1802-16) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. IHC-P
  2. IF-cell
  3. IF
  4. IHC

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