Mouse anti-Human IgG Mouse Monoclonal Antibody [A5D12]
Catalog# HA600076
Mouse anti-Human IgG Mouse Monoclonal Antibody [A5D12]
-
WB
-
ELISA
-
Human
-
unconjugated
概述
产品名称
Mouse anti-Human IgG Mouse Monoclonal Antibody [A5D12]
抗体类型
Mouse Monoclonal Antibody
免疫原
Full length native protein corresponding to Human IgG.
种属反应性
Human
验证应用
WB, ELISA
阳性对照
Human IgG protein.
偶联
unconjugated
克隆号
A5D12
RRID
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein G affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
-
ELISA
-
1:1,000-1:20,000
靶点
功能
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
背景文献
暂无
亚细胞定位
Cell Membrane and Secreted.
UNIPROT
别名
FLJ39988 antibody
FLJ40036 antibody
FLJ40253 antibody
FLJ40587 antibody
FLJ40789 antibody
FLJ40834 antibody
G1m marker antibody
G2m marker antibody
G3m marker antibody
G4m marker antibody
展开图片
-
Western blot analysis of Mouse anti-Human IgG on different lysates with Mouse anti-Mouse anti-Human IgG antibody (HA600076) at 1/1,000 dilution.
Lane 1: Recombinant human IgG protein
Lane 2: Recombinant human IgM protein
Lysates/proteins at 50 ng/Lane.
Observed band size: 50 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600076) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immobilized Human IgG protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600076) for 1 hour at 25℃.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号