LRRK2 Recombinant Rabbit Monoclonal Antibody [PSH07-96]
Catalog# HA722932
LRRK2 Recombinant Rabbit Monoclonal Antibody [PSH07-96]
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IHC-P
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IF-Tissue
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IHC-Fr
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Human
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Mouse
-
Rat
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Cynomolgus monkey
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Pig
预测的种属支持售后
概述
产品名称
LRRK2 Recombinant Rabbit Monoclonal Antibody [PSH07-96]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human LRRK2 aa 1,301-1,600.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
IHC-P, IF-Tissue, IHC-Fr
分子量
Predicted band size: 286 kDa
阳性对照
Human brain tissue, mouse brain tissue, rat brain tissue.
偶联
unconjugated
克隆号
PSH07-96
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-P
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1:1,000-1:10,000
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IF-Tissue
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1:200-1:1,000
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IHC-Fr
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1:200-1:500
靶点
功能
The LRRK2 gene encodes a protein with an armadillo repeats (ARM) region, an ankyrin repeat (ANK) region, a leucine-rich repeat (LRR) domain, a kinase domain, a RAS domain, a GTPase domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. LRRK2 interacts with the C-terminal R2 RING finger domain of parkin, and parkin interacted with the COR domain of LRRK2. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons. Expression of LRRK2 mutants implicated in autosomal dominant Parkinson's disease causes shortening and simplification of the dendritic tree in vivo and in cultured neurons. This is mediated in part by alterations in macroautophagy, and can be prevented by protein kinase A regulation of the autophagy protein LC3. The G2019S and R1441C mutations elicit post-synaptic calcium imbalance, leading to excess mitochondrial clearance from dendrites by mitophagy. LRRK2 is also a substrate for chaperone-mediated autophagy.
背景文献
1. Usmani A et al. The Cell Biology of LRRK2 in Parkinson\'s Disease. Mol Cell Biol. 2021 Apr
2. Rocha EM et al. LRRK2 and idiopathic Parkinson\'s disease. Trends Neurosci. 2022 Mar
亚细胞定位
Cytoplasmic vesicle, Perikaryon, Golgi apparatus membrane, Cell projection, axon, dendrite, Endoplasmic reticulum membrane, secretory vesicle, synaptic vesicle membrane, Endosome, Lysosome, Mitochondrion outer membrane, Cytoplasm, cytoskeleton.
别名
augmented in rheumatoid arthritis 17 antibody
AURA17 antibody
Dardarin antibody
Leucine rich repeat kinase 2 antibody
leucine rich repeat serine threonine protein kinase 2 antibody
Leucine-rich repeat serine/threonine-protein kinase 2 antibody
LRRK 2 antibody
LRRK2 antibody
LRRK2_HUMAN antibody
PARK 8 antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-LRRK2 antibody (HA722932) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722932) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LRRK2 antibody (HA722932) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722932) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LRRK2 antibody (HA722932) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722932) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-tissue
Species: Mouse
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1/200 -
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
LRRK2 Recombinant Rabbit Monoclonal Antibody [PSH07-96] - BSA and Azide free
Application: IHC-P,IF-Tissue,IHC-Fr
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
LRRK2 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P
Reactivity: Human,Mouse
Conjugate: unconjugated
