L1CAM Rabbit Polyclonal Antibody
Catalog# 0805-5
L1CAM Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
概述
产品名称
L1CAM Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within Human L1CAM aa 1,208-1,257 / 1,257.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 140 kDa
阳性对照
A375 cell lysate, HeLa cell lysate, human brain tissue lysate, rat brain tissue lysate, mouse brain tissue lysate, A375, 293T, Hela, human breast cancer tissue, human kidney tissue, mouse kidney tissue, SHG-44.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:600
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FC
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1:50-1:100
靶点
功能
The neural cell adhesion molecule L1 (NCAM-L1) is an immunoglobulin superfamily adhesion molecule that is critical for neural development, involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Defects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS). Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. Defects in L1CAM are also the cause of mental retardation, aphasia, shuffling gait, and adducted thumbs syndrome (MASA), a X-linked disorder.
背景文献
1. Fu TG et al. miR-143 inhibits oncogenic traits by degrading NUAK2 in glioblastoma. Int J Mol Med 37:1627-35 (2016).
2. Yoo M et al. Analysis of human embryonic stem cells with regulatable expression of the cell adhesion molecule l1 in regeneration after spinal cord injury. J Neurotrauma 31:553-64 (2014).
序列相似性
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
亚细胞定位
Cell membrane, Cell projection, Membrane.
别名
Antigen identified by monoclonal antibody
R1 antibody
CAML1 antibody
CD171 antibody
CD171 antigen antibody
HSAS antibody
HSAS1 antibody
Hyd antibody
L1 antibody
L1 cell adhesion molecule antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of L1CAM on different lysates with Rabbit anti-L1CAM antibody (0805-5) at 1/1,000 dilution.
Lane 1: A375 cell lysate
Lane 2: A549 cell lysate (low expression)
Lane 3: HeLa cell lysate
Lane 4: Human brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 140 kDa
Observed band size: 250 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0805-5) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of L1CAM on different lysates with Rabbit anti-L1CAM antibody (0805-5) at 1/1,000 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 140 kDa
Observed band size: 220 kDa
Exposure time: 2 minute;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0805-5) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A375 cells labeling L1CAM with Rabbit anti-L1CAM antibody (0805-5) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-L1CAM antibody (0805-5) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. -
ICC staining NCAML1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining NCAML1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-L1CAM antibody (0805-5) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0805-5) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NCAML1 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of SHG-44 cells with NCAML1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
L1CAM Recombinant Rabbit Monoclonal Antibody [JM11-05]
Application: WB,IHC-P,IF-Tissue
Reactivity: Human
Conjugate: unconjugated
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