KPNA2 Recombinant Rabbit Monoclonal Antibody [JM90-33]
Catalog# ET1705-61
KPNA2 Recombinant Rabbit Monoclonal Antibody [JM90-33]
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WB
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FC
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Human
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Mouse
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Rat
概述
产品名称
KPNA2 Recombinant Rabbit Monoclonal Antibody [JM90-33]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human KPNA2 aa 41-90 / 529.
种属反应性
Human, Mouse, Rat
验证应用
WB, FC
分子量
Predicted band size: 58 kDa
阳性对照
Hela cell lysate, 293T cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, 293T.
偶联
unconjugated
克隆号
JM90-33
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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FC
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1:50-1:100
靶点
功能
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
背景文献
1. Yang CP et al. Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58). J Biomed Sci 22:33 (2015).
2. Rachidi SM et al. Molecular profiling of multiple human cancers defines an inflammatory cancer-associated molecular pattern and uncovers KPNA2 as a uniform poor prognostic cancer marker. PLoS One 8:e57911 (2013).
序列相似性
Belongs to the importin alpha family.
组织特异性
Expressed ubiquitously.
亚细胞定位
Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus.
别名
IMA1_HUMAN antibody
Importin alpha 1 antibody
Importin alpha 2 antibody
Importin alpha 2 subunit antibody
Importin alpha P1 antibody
Importin subunit alpha-1 antibody
IPO A1 antibody
IPOA 1 antibody
IPOA1 antibody
Karyopherin alpha 2 antibody
展开图片
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Western blot analysis of KPNA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: HepG2 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate -
Flow cytometric analysis of KPNA2 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-61, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"