JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245] - BSA and Azide free
Catalog# HA750118
JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245] - BSA and Azide free
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WB
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IF-Cell
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Human
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Mouse
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Rat
概述
产品名称
JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within C-terminal human JAK2.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell
分子量
Predicted band size: 131 kDa
阳性对照
TF-1 cell lysate, K-562 cell lysate, THP-1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Jurkat cell lysate, MEF cell lysate, A549, MCF-7, NIH/3T3.
偶联
unconjugated
克隆号
SY0245
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:2,000
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IF-Cell
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1:100-1:500
靶点
功能
JAK2 (Janus kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.
背景文献
1. Gan, X.T. et al. 2015. Myocardial Hypertrophic Remodeling and Impaired Left Ventricular Function in Mice with a Cardiac-Specific Deletion of Janus Kinase 2. The American journal of pathology. 185: 3202-10.
2. Tang, H. et al. 2011. Changes in growth hormone (GH), GH receptor, and GH signal transduction in hippocampus of congenital hypothyroid rats. J. Neurosci. Res. 89: 248-255.
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
组织特异性
Ubiquitously expressed throughout most tissues.
翻译后修饰
Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 (By similarity). Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity (By similarity). Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity (By similarity). Also phosphorylated by TEC (By similarity). Phosphorylated on tyrosine residues in response to interferon gamma signaling. Phosphorylated on tyrosine residues in response to a signaling cascade that is activated by increased cellular retinol.
亚细胞定位
Endomembrane system, Cytoplasm, Nucleus.
别名
JAK 2 antibody
JAK-2 antibody
JAK2 antibody
JAK2_HUMAN antibody
Janus Activating Kinase 2 antibody
Janus kinase 2 (a protein tyrosine kinase) antibody
Janus kinase 2 antibody
JTK 10 antibody
JTK10 antibody
kinase Jak2 antibody
展开图片
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Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (HA750118) at 1/2,000 dilution.
Lane 1: TF-1 cell lysate
Lane 2: K-562 cell lysate
Lane 3: THP-1 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 131 kDa
Observed band size: 120 kDa
Exposure time: 5 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (HA750118) at 1/2,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si JAK2 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 131 kDa
Observed band size: 120 kDa
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
ICC staining JAK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining JAK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining JAK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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