Integrin beta 5 Recombinant Rabbit Monoclonal Antibody [PSH10-13] - BSA and Azide free

Western blot analysis of Integrin beta 5 on different lysates with Rabbit anti-Integrin beta 5 antibody (HA751342) at 1/2,000 dilution.

Lane 1: A549 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: NCI-H1299 cell lysate
Lane 4: PANC-1 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: C6 cell lysate
Lane 7: Rat lung tissue lysate
Lane 8: Rat kidney tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 88 kDa
Observed band size: 88 kDa

Exposure time: 1 minute 20 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751342) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Integrin beta 5 antibody (HA751342) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA751342) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Integrin beta 5 antibody (HA751342) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA751342) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.