Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [SJ19-09]

Western blot analysis of Integrin beta 3 on U-87 MG cell lysates with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/2,000 dilution.

Lysates/proteins at 15 µg/Lane.

Predicted band size: 87 kDa
Observed band size: 100 kDa

Exposure time: 1 minute 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-49) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

☑ Knockdown (KD)

Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/500 dilution.

Lane 1: U87-MG-si NT cell lysate
Lane 2: U87-MG-si Integrin beta 3 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 87 kDa
Observed band size: 87,100 kDa

Exposure time: 1 minutes 10 seconds;

4-20% SDS-PAGE gel.

ET1606-49 was shown to specifically react with Integrin beta 3 in U87-MG-si NT cells. Weakened band was observed when U87-MG-si Integrin beta 3 sample was tested. U87-MG-si NT and U87-MG-si Integrin beta 3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-49, 1/500) and Loading control antibody (Rabbit anti-Hsp90, ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1606-49) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1606-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.