Human Adiponectin Recombinant Rabbit Monoclonal Antibody [PSH05-32] - BSA and Azide free (Detector)
概述
产品名称
Human Adiponectin Recombinant Rabbit Monoclonal Antibody [PSH05-32] - BSA and Azide free (Detector)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Adiponectin aa 19-244 (Q15848).
种属反应性
Human
验证应用
ELISA(Det)
阳性对照
Recombinant standard Human Adiponectin protein (HA210935).
偶联
unconjugated
克隆号
PSH05-32
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-31] to Human Adiponectin (Capture) (HA722260) and Recombinant Human Adiponectin protein (HA210935) as the standard. The reference range value is 61.7-5000pg/ml.
靶点
功能
Adiponectin (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein hormone and adipokine, which is involved in regulating glucose levels and fatty acid breakdown. In humans, it is encoded by the ADIPOQ gene and is produced primarily in adipose tissue, but also in muscle and even in the brain. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Adiponectin is secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones. High adiponectin levels correlate with a lower risk of diabetes mellitus type 2. Plasma levels of adiponectin are lower in obese subjects than in lean subjects. Many studies have found adiponectin to be inversely correlated with body mass index in patient populations. However, a meta analysis was not able to confirm this association in healthy adults. Circulating adiponectin concentrations increase during caloric restriction in animals and humans, such as in patients with anorexia nervosa. Furthermore, a recent study suggests that adipose tissue within bone marrow, which increases during caloric restriction, contributes to elevated circulating adiponectin in this context.
背景文献
1. Zhao S et al. Adiponectin, Leptin and Cardiovascular Disorders. Circ Res. 2021 Jan
2. Choi HM et al. Multifaceted Physiological Roles of Adiponectin in Inflammation and Diseases. Int J Mol Sci. 2020 Feb
亚细胞定位
Secreted.
UNIPROT
别名
30 kDa adipocyte complement related protein antibody
30 kDa adipocyte complement-related protein antibody
ACDC antibody
Acrp 30 antibody
ACRP30 antibody
ADIPO_HUMAN antibody
Adipocyte antibody
Adipocyte C1q and collagen domain containing protein antibody
Adipocyte complement related 30 kDa protein antibody
Adipocyte complement related protein of 30 kDa antibody
展开图片
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Sandwich ELISA analysis of Human adiponectin matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722260) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant standard Human Adiponectin protein (HA210935) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA722261, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of spiked Adiponectin in human cell culture media samples.
The concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
Interpolated concentration of native Adiponectin was measured in duplicate at different sample concentrations. Undiluted samples were 1:20000 serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Biotin Conjugated Human Adiponectin Recombinant Rabbit Monoclonal Antibody [PSH05-32] - Detector
Application: ELISA(Det),ELISA
Reactivity: Human
Conjugate: Biotin
