Glucose 6 Phosphate Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JA31-40]
Catalog# ET1704-39
Glucose 6 Phosphate Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JA31-40]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
概述
产品名称
Glucose 6 Phosphate Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JA31-40]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 460-509 / 515.
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 59 kDa
阳性对照
A549 cell lysate, Hela, HepG2, MCF-7, human liver tissue, human stomach cancer tissue, mouse testes tissue, mouse skeletal muscle tissue.
偶联
unconjugated
克隆号
JA31-40
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
靶点
功能
Glucose-6-phosphate 1-dehydrogenase (G6PD) plays an important role in the pentose phosphate pathway. It is a member of the glucose-6-phosphate dehydrogenase family of proteins. G6PD is a ubiquitous enzyme that produces pentose sugars for nucleic acid synthesis, but is also involved in carbohydrate degradation, as it is one of the main producers of NADPH reducing power. G6PD has NADP as a co-factor and structural element. It can be found as a homodimer or homotetramer, and is primarily detected in lymphoblasts, granulocytes and sperm. Defects in G6PD can cause chronic non-spherocytic hemolytic anemia (CNSHA), especially in areas in which malaria is an epidemic. Individuals with a high level of G6PD-deficiency are at higher risk of acute hemolytic attacks.
背景文献
1. Kaneko Y et al.Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro.Sci Rep
序列相似性
Belongs to the glucose-6-phosphate dehydrogenase family.
组织特异性
Isoform Long is found in lymphoblasts, granulocytes and sperm.
翻译后修饰
Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
亚细胞定位
Cytoplasm. Cytoplasmic side of plasma membrane. Membrane. Nucleus.
UNIPROT
别名
G6PD antibody
G6PD_HUMAN antibody
G6PD1 antibody
G6pdx antibody
Glucose 6 phosphate 1 dehydrogenase antibody
Glucose 6 phosphate dehydrogenase antibody
Glucose 6 phosphate dehydrogenase, G6PD antibody
Glucose-6-phosphate 1-dehydrogenase antibody
MET19 antibody
POS10 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of Glucose 6 Phosphate Dehydrogenase on different lysates with Rabbit anti-Glucose 6 Phosphate Dehydrogenase antibody (ET1704-39) at 1/1,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si G6PD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-39) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Glucose 6 Phosphate Dehydrogenase on A549 cell lysate using anti-Glucose 6 Phosphate Dehydrogenase antibody at 1/1,000 dilution.
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ICC staining Glucose 6 Phosphate Dehydrogenase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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SILAC Quantitative Proteomics and Biochemical Analyses Reveal a Novel Molecular Mechanism by Which ADAM12S Promotes the Proliferation, Migration, and Invasion of Small Cell Lung Cancer Cells through Upregulating Hexokinase 1
期刊: Journal Of Proteome Research
DOI: 10.1021/acs.jproteome.9b00208
IF: 3.78
应用: WB
反应种属: Human
发表时间: 2019 May
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