DFNA5 / GSDME Recombinant Rabbit Monoclonal Antibody [PSH10-84] - BSA and Azide free
Catalog# HA751376
DFNA5 / GSDME Recombinant Rabbit Monoclonal Antibody [PSH10-84] - BSA and Azide free
-
WB
-
IP
-
Human
-
Mouse
-
HA723251
含抗保成分
-
unconjugated
概述
产品名称
DFNA5 / GSDME Recombinant Rabbit Monoclonal Antibody [PSH10-84] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human GSDME aa 1-496.
种属反应性
Human, Mouse
验证应用
WB, IP
分子量
Predicted band size: 55 kDa
阳性对照
SH-SY5Y cell lysate, SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate.
偶联
unconjugated
克隆号
PSH10-84
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IP
-
1-2μg/sample
靶点
功能
Non-syndromic hearing impairment protein 5 is a protein that in humans is encoded by the DFNA5 gene. Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. The observation that DFNA5 is epigenetically inactivated in a large number of cancers of frequent types (gastric, colorectal, and breast) is another important finding and is in line with its apoptosis-inducing properties. Indeed, if apoptosis is an intrinsic feature of DFNA5, shutting the gene down in tumor cells makes them more susceptible to uncontrolled cellular growth. Moreover, the fact that DFNA5 is regulated by P53 strongly suggests that DFNA5 is a tumor suppressor gene.
背景文献
1. Wei Y et al. GSDME-mediated pyroptosis promotes the progression and associated inflammation of atherosclerosis. Nat Commun. 2023 Feb
2. Ai YL et al. Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P. Cell Res. 2023 Dec
亚细胞定位
Cytoplasm, cytosol.
别名
2310037D07Rik antibody
4932441K13Rik antibody
Deafness, autosomal dominant 5 antibody
Deafness, autosomal dominant 5 protein antibody
DFNA5 antibody
DFNA5 gene antibody
DFNA5_HUMAN antibody
Dfna5h antibody
EG14210 antibody
Fin15 antibody
展开图片
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☑ Cell treatment (CT),Relative expression (RE)
Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751376) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate
Lane 3: SW480 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55/30 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751376) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751376) at 1/2,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with mouse GSDME (N-terminal) cell lysate
Lane 3: 293T transfected with mouse GSDME (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751376) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA751376 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751376 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input)
Lane 2: HA751376 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate
Lane 3: Rabbit IgG instead of HA751376 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 10 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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