DDX58 Recombinant Rabbit Monoclonal Antibody [JE60-05]
Catalog# HA720090
DDX58 Recombinant Rabbit Monoclonal Antibody [JE60-05]
-
WB
-
Human
概述
产品名称
DDX58 Recombinant Rabbit Monoclonal Antibody [JE60-05]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human DDX58 aa 250-450/925.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 107 kDa
阳性对照
HUVEC cell lysates.
偶联
unconjugated
克隆号
JE60-05
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500
靶点
功能
Innate immune receptor that senses cytoplasmic viral nucleic acids and activates a downstream signaling cascade leading to the production of type I interferons and proinflammatory cytokines. Forms a ribonucleoprotein complex with viral RNAs on which it homooligomerizes to form filaments. The homooligomerization allows the recruitment of RNF135 an E3 ubiquitin-protein ligase that activates and amplifies the RIG-I-mediated antiviral signaling in an RNA length-dependent manner through ubiquitination-dependent and -independent mechanisms. Upon activation, associates with mitochondria antiviral signaling protein (MAVS/IPS1) that activates the IKK-related kinases TBK1 and IKBKE which in turn phosphorylate the interferon regulatory factors IRF3 and IRF7, activating transcription of antiviral immunological genes including the IFN-alpha and IFN-beta interferons.
背景文献
1. Xian H. et. al. LRRC59 modulates type I interferon signaling by restraining the SQSTM1/p62-mediated autophagic degradation of pattern recognition receptor DDX58/RIG-I. Autophagy. 2020 Mar
2. Ferreira CR. et. al. DDX58 and Classic Singleton-Merten Syndrome. J Clin Immunol. 2019 Jan
亚细胞定位
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Tight junction.
UNIPROT
别名
Ddx58 antibody
DDX58_HUMAN antibody
DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody
DEAD (Asp Glu Ala Asp/His) box polypeptide antibody
DEAD box protein 58 antibody
DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody
DKFZp434J1111 antibody
DKFZp686N19181 antibody
FLJ13599 antibody
Probable ATP dependent RNA helicase DDX58 antibody
展开图片
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Western blot analysis of DDX58 on HUVEC cell lysates with Rabbit anti-DDX58 antibody (HA720090) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720090) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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