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CD1a Recombinant Rabbit Monoclonal Antibody [JE28-74]

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Catalog# ET7110-48

CD1a Recombinant Rabbit Monoclonal Antibody [JE28-74]

Application

  • WB

  • IHC-P

Reactivity

  • Human

概述

产品名称

CD1a Recombinant Rabbit Monoclonal Antibody [JE28-74]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human CD1a aa 1-50 / 327.

种属反应性

Human

验证应用

WB, IHC-P

分子量

Predicted band size: 37 kDa

阳性对照

Human skin tissue lysate, human thymus tissue lysate, human skin tissue.

偶联

unconjugated

克隆号

JE28-74

RRID

AB_3071013

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

靶点

功能

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternatively spliced transcript variants have been observed, but their biological validity has not been determined.

背景文献

1. Al-Amodi O. et. al. CD1a-Expressing Monocytes as Mediators of Inflammation in Ulcerative Colitis. Inflamm Bowel Dis. 2018 May 17.

2. Carrera Silva EA. et. al. CD207+CD1a+ cells circulate in pediatric patients with active Langerhans cell histiocytosis. Blood. 2017 Oct 26;130(17):1898-1902.

组织特异性

Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.

亚细胞定位

Endosome membrane, cell membrane, membrane raft.

UNIPROT

SwissProt: P06126 Human

别名

CD 1a antibody

CD1 antibody

CD1a antibody

CD1A Antigen antibody

CD1A antigen, a polypeptide antibody

CD1a molecule antibody

CD1A_HUMAN antibody

cluster of differentiation 1 A antibody

cortical thymocyte antigen CD1A antibody

differentiation antigen CD1 alpha 3 antibody

展开

图片

  • Western blot analysis of CD1a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-48" style="font-weight: bold;text-decoration: underline;">ET7110-48</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: Human skin tissue lysate<br />Lane 2: Human thymus tissue lysate

    Western blot analysis of CD1a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Human skin tissue lysate
    Lane 2: Human thymus tissue lysate

  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CD1a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-48" style="font-weight: bold;text-decoration: underline;">ET7110-48</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CD1a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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