CD134 Recombinant Rabbit Monoclonal Antibody [JE36-48]
Catalog# HA722700
CD134 Recombinant Rabbit Monoclonal Antibody [JE36-48]
-
WB
-
Human
概述
产品名称
CD134 Recombinant Rabbit Monoclonal Antibody [JE36-48]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human CD134 aa 1-250 / 277.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 29 kDa
阳性对照
HUT 102 cell lysate.
偶联
unconjugated
克隆号
JE36-48
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor, is a member of the TNFR-superfamily of receptors which is not constitutively expressed on resting naïve T cells, unlike CD28. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. Expression of OX40 is dependent on full activation of the T cell; without CD28, expression of OX40 is delayed and of fourfold lower levels.
背景文献
1. Kwiatek M et al. OX40 (CD134) Expression on T Regulatory Cells Is Related to Serious Hypertensive Disorders in Pregnancy. J Cardiovasc Dev Dis. 2023 Oct
2. Ansari AW et al. Azithromycin downregulates ICOS (CD278) and OX40 (CD134) expression and mTOR activity of TCR-activated T cells to inhibit proliferation. Int Immunopharmacol. 2023 Nov
亚细胞定位
Membrane.
UNIPROT
别名
ACT 35 antibody
ACT35 antibody
ACT35 antigen antibody
ATC35 antigen antibody
CD 134 antibody
CD134 antibody
CD134 antigen antibody
IMD16 antibody
Lymphoid activation antigene ACT35 antibody
OX 40 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of CD134 on different lysates with Rabbit anti-CD134 antibody (HA722700) at 1/1,000 dilution.
Lane 1: HUT 102 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 50 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
CD134 Recombinant Rabbit Monoclonal Antibody [PSH10-14] - BSA and Azide free
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Reactivity: Human
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CD134 Recombinant Rabbit Monoclonal Antibody [PSH10-14]
Application: WB,IF-Cell,FC,IP
Reactivity: Human
Conjugate: unconjugated
