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Bcl-2 Mouse Monoclonal Antibody [9F1]

RMB: 900 特惠 1500

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Catalog# EM1701-82

Bcl-2 Mouse Monoclonal Antibody [9F1]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Bcl-2 Mouse Monoclonal Antibody [9F1]

抗体类型

Mouse Monoclonal Antibody

免疫原

Synthetic peptide within human BCL2 35-60 aa.

种属反应性

Human

验证应用

WB, IHC-P

分子量

Predicted band size: 26 kDa

阳性对照

THP-1 cell lysates, human tonsil tissue.

偶联

unconjugated

克隆号

9F1

RRID

AB_3068806

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:500-1:1,000

靶点

功能

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.

背景文献

1. Yin X-M et al. BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. Nature 369:321-323 (1994).

2. Naumovski L et al. The p53-binding protein 53BP2 also interacts with Bcl2 and impedes cell cycle progression at G2/M. Mol Cell Biol 16:3884-3892 (1996).

序列相似性

Belongs to the Bcl-2 family.

组织特异性

Expressed in a variety of tissues.

翻译后修饰

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

亚细胞定位

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

UNIPROT

SwissProt: P10415 Human

别名

Apoptosis regulator Bcl 2 antibody

Apoptosis regulator Bcl-2 antibody

Apoptosis regulator Bcl2 antibody

AW986256 antibody

B cell CLL/lymphoma 2 antibody

B cell leukemia/lymphoma 2 antibody

Bcl-2 antibody

Bcl2 antibody

BCL2_HUMAN antibody

C430015F12Rik antibody

展开

图片

  • Western blot analysis of Bcl-2 on THP-1 cell lysates with Mouse anti-Bcl-2 antibody (<a href="/products/EM1701-82" style="font-weight: bold;text-decoration: underline;">EM1701-82</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 1 minute;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-82" style="font-weight: bold;text-decoration: underline;">EM1701-82</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Bcl-2 on THP-1 cell lysates with Mouse anti-Bcl-2 antibody (EM1701-82) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 1 minute;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-82) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BCL2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BCL2 antibody. Counter stained with hematoxylin.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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