Acid phosphatase/ACP1 Recombinant Rabbit Monoclonal Antibody [PSH04-57]
Catalog# HA722156
Acid phosphatase/ACP1 Recombinant Rabbit Monoclonal Antibody [PSH04-57]
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WB
-
IHC-P
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Human
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Green monkey
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HA750964
不含抗保成分
-
unconjugated
概述
产品名称
Acid phosphatase/ACP1 Recombinant Rabbit Monoclonal Antibody [PSH04-57]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human ACP1 aa 1-158 / 158.
种属反应性
Human, Green monkey
验证应用
WB, IHC-P
分子量
Predicted band size: 18 kDa
阳性对照
HepG2 cell lysate, HeLa cell lysate, Jurkat cell lysate, COS-1 cell lysate, human kidney tissue, human stomach tissue, human testis tissue.
偶联
unconjugated
克隆号
PSH04-57
反应性数据
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Green Monkey | ||
| Monkey |
|
|
| Mouse |
|
|
| Rat |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:1,000
靶点
功能
Low molecular weight phosphotyrosine protein phosphatase is an enzyme that in humans is encoded by the ACP1 gene. The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene.
背景文献
1. Feng B et al. Discovery and biological evaluation of novel dual PTP1B and ACP1 inhibitors for the treatment of insulin resistance. Bioorg Med Chem. 2024 Jan
2. Abdelgalil SA et al. A sustainable and effective bioprocessing approach for improvement of acid phosphatase production and rock phosphate solubilization by Bacillus haynesii strain ACP1. Sci Rep. 2022 May
亚细胞定位
Cytoplasm.
UNIPROT
别名
Acid phosphatase 1 soluble antibody
Acid phosphatase of erythrocyte antibody
ACP1 antibody
Adipocyte acid phosphatase antibody
Cytoplasmic phosphotyrosyl protein phosphatase antibody
HAAP antibody
LMW-PTP antibody
LMW-PTPase antibody
Low molecular weight cytosolic acid phosphatase antibody
Low molecular weight phosphotyrosine protein phosphatase antibody
展开图片
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Western blot analysis of Acid phosphatase/ACP1 on different lysates with Rabbit anti-Acid phosphatase/ACP1 antibody (HA722156) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 1 minute 33 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722156) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Acid phosphatase/ACP1 antibody (HA722156) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Acid phosphatase/ACP1 antibody (HA722156) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Acid phosphatase/ACP1 antibody (HA722156) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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