ATG4B Recombinant Rabbit Monoclonal Antibody [PSH02-53]
Catalog# HA721829
ATG4B Recombinant Rabbit Monoclonal Antibody [PSH02-53]
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WB
-
Human
-
Mouse
-
Rat
-
Monkey
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HA750816
不含抗保成分
-
unconjugated
概述
产品名称
ATG4B Recombinant Rabbit Monoclonal Antibody [PSH02-53]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human ATG4B aa 1-393 / 393 (Q9Y4P1).
种属反应性
Human, Mouse, Rat, Monkey
验证应用
WB
分子量
Predicted band size: 44 kDa
阳性对照
HeLa cell lysate, Jurkat cell lysate, 293T cell lysate, MCF7 cell lysate, K-562 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Mouse kidney tissue lysate, Mouse colon tissue lysate.
偶联
unconjugated
克隆号
PSH02-53
RRID
反应性数据
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
| Monkey |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
靶点
功能
Cysteine protease ATG4B is an enzyme that in humans is encoded by the ATG4B gene. Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. One main function of Atg4 is to cleave the pre-protein of Atg8, leading to the non-lipidated soluble (-I) form which can be processed further by Atg3, Atg7, Atg5-12 into the lipidated form (-II) anchored to the autophagic membrane.
背景文献
1. Sun L et al. Deacetylation of ATG4B promotes autophagy initiation under starvation. Sci Adv. 2022 Aug
2. Yang G et al. Targeting Atg4B for cancer therapy: Chemical mediators. Eur J Med Chem. 2021 Jan
亚细胞定位
Cytoplasm, cytosol, Cytoplasmic vesicle, autophagosome, Endoplasmic reticulum, Mitochondrion.
别名
APG4 autophagy 4 homolog B antibody
APG4B antibody
ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
ATG4B antibody
ATG4B_HUMAN antibody
AUT like 1 cysteine endopeptidase antibody
AUT-like 1 cysteine endopeptidase antibody
AUTL1 antibody
Autophagin 1 antibody
Autophagin-1 antibody
展开图片
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Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (HA721829) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 293T cell lysate
Lane 4: MCF7 cell lysate
Lane 5: K-562 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: C2C12 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: Mouse brain tissue lysate
Lane 11: Rat brain tissue lysate
Lane 12: Mouse kidney tissue lysate
Lane 13: Mouse colon tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 47 kDa
Exposure time: 42 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721829) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (HA721829) at 1/2,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: COS-1 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 47 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721829) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (HA721829) at 1/1,000 dilution.
Lane 1: 293T-si NT cell lysate
Lane 2: 293T-si ATG4B cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 47 kDa
Exposure time: 51 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721829) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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