• 概述

• 产品描述 Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.
• 产品名称 Anti-beta Tubulin Recombinant Rabbit Monoclonal Antibody [SR25-04]
• 分子量 Predicted band size: 50 kDa
• 种属反应性 Human,Mouse,Rat
• 验证应用 WB,ICC,IHC-P,FC
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within Human beta Tubulin aa 308-357 / 444.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cytoplasm
• 数据链接 SwissProt: P07437 Human
  SwissProt: P99024 Mouse
  SwissProt: P69897 Rat
  
• 其它名称
  • Beta 4 tubulin antibody
  • Beta 5 tubulin antibody
  • BetaTubulin antibody
  • TBB5_HUMAN antibody
  • TUBB antibody
  • TUBB2 antibody
  • TUBB2A antibody
  • TUBB5 antibody
  • tubulin beta 2A antibody
  • Tubulin beta chain antibody
  • Tubulin beta-5 chain antibody
  more

• 应用

  WB: 1:500
  ICC: 1:50
  IHC-P: 1:100-400
  FC: 1:50

• 

  Fig1: Western blot analysis of beta Tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: A431 cell lysate
  Lane 2: MCF-7 cell lysate
  Lane 3: SH-SY5Y cell lysate
  Lane 4: mouse brain tissue lysate
  Lane 5: Hela cell lysate
  Lane 6: NIH/3T3 cell lysate
  Lane 7: PC-12 cell lysate

  

  Fig2: ICC staining of beta Tubulin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining of beta Tubulin in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: ICC staining of beta Tubulin in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig5: ICC staining of beta Tubulin in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig6: ICC staining of beta Tubulin in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/100 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-4) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig8: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/400 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-4) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig9: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/400 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-4) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig10: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/400 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-4) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig11: Flow cytometric analysis of beta Tubulin was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1602-4, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

• 背景文献

• 1. "Tumoral and tissue-specific expression of the major human beta-tubulin isotypes."Leandro-Garcia L.J., Leskela S., Landa I., Montero-Conde C., Lopez-Jimenez E., Leton R., .Cytoskeleton 67:214-223(2010).
  
  2. "Five mouse tubulin isotypes and their regulated expression during development."Lewis S.A., Lee M.G.-S., Cowan N.J.J. Cell Biol. 101:852-861(1985).

• Publishing research using Anti-beta Tubulin Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  35408869 Toward a Treatment of Cancer: Design and In Vitro/In Vivo Evaluation of Uncharged Pyrazoline Derivatives as a Series of Novel SHP2 Inhibitors Author: Dai, J., Zhang, Y., Gao, Y., Bai, X., Liu, F., Li, S., Yu, Y., Hu, W., Shi, T., Shi, D., & Li, X. Published: 2022 Application: WB Species: Human Journal: Int J Mol Sci IF: 5.924

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.