• 概述

• 产品描述 UGGT is part of the ER quality control system of glycoprotein folding and its activity increases the potential for correctly folded glycoproteins.The main proteins involved in the ER quality control system are UGGT, the ER lectin chaperones (calnexin and calreticulin), and glucosidase II. UGGT first recognizes the incompletely folded glycoprotein and monoglucosylates it. The lectins, calnexin and calreticulin, have high affinities for monoglucosylated proteins and the ER chaperones that associate with these lectins assist the folding of the misfolded glycoprotein. Subsequently, glucosidase II will deglucosylate the glycoprotein. If the glycoprotein is still misfolded, UGGT will re-glucosylate it and allow it to go through the cycle again.
• 产品名称 Anti-UGGT1 antibody
• 分子量 177 kDa
• 种属反应性 Human,Mouse,Rat
• 验证应用 WB,IHC-P
• 抗体类型 兔多抗
• 免疫原 Recombinant protein within human UGGT1 aa 1350-1555.
• RRID AB_3071125

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Protein affinity purified.
• 亚细胞定位 Endoplasmic reticulum.
• 数据链接 SwissProt: Q9NYU2 Human
  SwissProt: Q6P5E4 Mouse
  SwissProt: Q9JLA3 Rat
  
• 其它名称
  • GT antibody
  • HUGT1 antibody
  • UDP glucose glycoprotein glucosyltransferase 1 antibody
  • UDP Glucose Glycoprotein Glucosyltransferase antibody
  • UDP--Glc:glycoprotein glucosyltransferase antibody
  • UDP-glucose ceramide glucosyltransferase-like 1 antibody
  • UDP-glucose:glycoprotein glucosyltransferase 1 antibody
  • UGCGL1 antibody
  • UGGG1 antibody
  • UGGG1_HUMAN antibody
  • UGGT antibody
  • Uggt1 antibody
  • UGT1 antibody
  • UGTR antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:50-1:200

• 

  Fig1: Western blot analysis of UGGT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500025, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Rat liver tissue lysate
  Lane 2: HL-60 cell lysate
  Lane 3: Mouse liver tissue lysate

  

  Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-UGGT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500025, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• 背景文献

• 1. Arnold S.M. et. al. Two homologues encoding human UDP-glucose:glycoprotein glucosyltransferase differ in mRNA expression and enzymatic activity. Biochemistry 39:2149-2163(2000).

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.