• 概述

• 产品描述 Transforming growth factor beta-1: Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1. Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (PubMed:20207738).
• 产品名称 Anti-TGF beta 1 Rabbit Polyclonal Antibody
• 分子量 Predicted band size: 44 kDa
• 种属反应性 Human,Mouse,Rat,Zebrafish
• 验证应用 WB,IF-Cell,IHC-P,FC
• 抗体类型 兔多抗
• 免疫原 Synthetic peptide (KLH-coupled) corresponding to Human TGF-Beta 1 C-terminal.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Immunogen affinity purified.
• 亚细胞定位 Secreted
• 数据链接 SwissProt: P01137 Human
  SwissProt: P04202 Mouse
  SwissProt: P17246 Rat
  
• 其它名称
  • Cartilage-inducing factor antibody
  • CED antibody
  • Differentiation inhibiting factor antibody
  • DPD1 antibody
  • LAP antibody
  • Latency-associated peptide antibody
  • Prepro transforming growth factor beta 1 antibody
  • TGF beta 1 antibody
  • TGF beta antibody
  • TGF beta 1 protein antibody
  • TGF-beta 1 protein antibody
  • TGF-beta-1 antibody
  • TGF-beta-5 antibody
  • TGF-beta1 antibody
  • TGFB antibody
  • Tgfb-1 antibody
  • tgfb1 antibody
  • TGFB1_HUMAN antibody
  • TGFbeta antibody
  • TGFbeta1 antibody
  • Transforming Growth Factor b1 antibody
  • Transforming Growth Factor beta 1 antibody
  • Transforming growth factor beta 1a antibody
  • transforming growth factor beta-1 antibody
  • transforming growth factor, beta 1 antibody
  • Transforming Growth Factor-ß1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IF-Cell: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of TGF beta 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER31210, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Raji cell lysate
  Lane 2: MCF-7 cell lysate
  Lane 3: A549 cell lysate
  Lane 4: Human kidney tissue lysate

  

  Fig2: ICC staining of TGF beta 1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER31210, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining of TGF beta 1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER31210, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: ICC staining of TGF beta 1 in SK-Br-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER31210, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF beta 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER31210, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TGF beta 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER31210, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig7: Flow cytometric analysis of TGF beta 1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER31210, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

• 背景文献

• 1. "A mutation affecting the latency-associated peptide of TGFbeta1 in Camurati-Engelmann disease enhances osteoclast formation in vitro." McGowan N.W., MacPherson H., Janssens K., Van Hul W., Frith J.C., Fraser W.D., Ralston S.H., Helfrich M.H. J. Clin. Endocrinol. Metab. 88:3321-3326(2003)
  
  2. "Identification of CD109 as part of the TGF-beta receptor system in human keratinocytes." Finnson K.W., Tam B.Y.Y., Liu K., Marcoux A., Lepage P., Roy S., Bizet A.A., Philip A. FASEB J. 20:1525-1527(2006)
  
  3. "Loss-of-function mutations in TGFB2 cause a syndromic presentation of thoracic aortic aneurysm." Lindsay M.E., Schepers D., Bolar N.A., Doyle J.J., Gallo E., Fert-Bober J., Kempers M.J., Fishman E.K., Chen Y., Myers L., Bjeda D., Oswald G., Elias A.F., Levy H.P., Anderlid B.M., Yang M.H., Bongers E.M., Timmermans J. , Braverman A.C., Canham N., Mortier G.R., Brunner H.G., Byers P.H., Van Eyk J., Van Laer L., Dietz H.C., Loeys B.L. Nat. Genet. 44:922-927(2012)

• Publishing research using Anti-TGF beta 1 Rabbit Polyclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  35202887 Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine–Induced Acute Liver Failure Through Inhibiting GSK3β–Nrf2–Mediated Hepatocyte Apoptosis and Ferroptosis Author: Huang, S., Wang, Y., Xie, S., Lai, Y., Mo, C., Zeng, T., Kuang, S., Deng, G., Zhou, C., Chen, Y., Huang, S., Gao, L., & Lv, Z. Published: 2022 Application: IF Species: Mouse Journal: Cell Mol Gastroenterol Hepatol IF: 9.225
• PMID:
  33744278 TGF-β1-induced EMT activation via both Smad-dependent and MAPK signaling pathways in Cu-induced pulmonary fibrosis Author: Guo, H., Jian, Z., Liu, H., Cui, H., Deng, H., Fang, J., Zuo, Z., Wang, X., Zhao, L., Geng, Y., Ouyang, P., & Tang, H. Published: 2021 Application: WB Species: Mouse Journal: Toxicol Appl Pharmacol IF: 4.211

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.