Synaptopodin Rabbit Polyclonal Antibody
Catalog# ER1917-40
Synaptopodin Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Tissue
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FC
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Human
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Mouse
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Rat
概述
产品名称
Synaptopodin Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human Synaptopodin 601-700/903
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue, FC
分子量
102 KDa
阳性对照
Expressed in brain, namely in the olfactory bulb, cerebral cortex, striatum, and hippocampus, but not in the cerebellum. Also expressed in the podocytes of kidney glomeruli. In the hippocampus, mainly expressed in the principal cell layer of the dentate gyrus and Ammon's horn.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2000
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IHC-P
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1:100-500
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IF-tissue
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1:100-500
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FC
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1μg /test
靶点
背景文献
暂无
亚细胞定位
Cytoplasm, cytoskeleton (By similarity). Cell junction, tight junction (By similarity). Perikaryon (By similarity). Cell projection, dendritic spine (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell junction, synapse (By similarity). Note=Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses (By similarity).
UNIPROT
别名
KIAA1029 antibody
Synaptopodin antibody
SYNPO antibody
SYNPO_HUMAN antibody
图片
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Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-Synaptopodin (ER1917-40) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 102 kD
Observed band size: 102 kD -
Sample: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Synaptopodin (ER1917-40) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 102 kD
Observed band size: 76 kD -
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Synaptopodin) Polyclonal Antibody, Unconjugated (ER1917-40) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Synaptopodin) Polyclonal Antibody, Unconjugated (ER1917-40) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Synaptopodin Recombinant Rabbit Monoclonal Antibody [PSH10-45]
Application: WB,IHC-Fr,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
同靶点 & 同通路的产品
Synaptopodin Recombinant Antibody [PSH10-45] - Rat IgG1 (Chimeric)
Application: IHC-Fr,IHC-P,WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated