Sall4 Mouse Monoclonal Antibody [A7A5]
Catalog# HA601041
Sall4 Mouse Monoclonal Antibody [A7A5]
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WB
-
IHC-P
-
Human
概述
产品名称
Sall4 Mouse Monoclonal Antibody [A7A5]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human Sall4 aa 903-1,053/1,053
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 112 kDa
阳性对照
NCCIT cell lysates, human seminoma tissue.
偶联
unconjugated
克隆号
A7A5
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
-
WB
-
1:2,000
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IHC-P
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1:600
靶点
功能
Sal-like protein 4 (SALL4) is a transcription factor encoded by a member of the Spalt-like (SALL) gene family, SALL4. The SALL genes were identified based on their sequence homology to Spalt, which is a homeotic gene originally cloned in Drosophila melanogaster that is important for terminal trunk structure formation in embryogenesis and imaginal disc development in the larval stages. There are four human SALL proteins (SALL1, 2, 3, and 4) with structural homology and playing diverse roles in embryonic development, kidney function, and cancer. The SALL4 gene encodes at least three isoforms, termed A, B, and C, through alternative splicing, with the A and B forms being the most studied. SALL4 can alter gene expression changes through its interaction with many co-factors and epigenetic complexes. It is also known as a key embryonic stem cell (ESC) factor. The various SALL4-null mouse models mimic human mutations in the SALL4 gene, which were shown to cause developmental problems in patients with Okihiro/Duane-Radial-ray syndrome. These individuals frequently have family history of hand malformation and eye movement disorders.
背景文献
1. Zhang X. et. al. SALL4 activates TGF-beta/SMAD signaling pathway to induce EMT and promote gastric cancer metastasis. Cancer Manag Res. 2018 Oct
2. Chen M. et. al. SALL4 promotes the tumorigenicity of cervical cancer cells through activation of the Wnt/beta-catenin pathway via CTNNB1. Cancer Sci. 2019 Sep
亚细胞定位
Cytoplasm, Nucleus.
UNIPROT
别名
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dJ1112F19.1 antibody
DRRS antibody
HSAL4 antibody
Sal like 4 (Drosophila) antibody
展开图片
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Western blot analysis of Sall4 on NCCIT cell lysates with Mouse anti-Sall4 antibody (HA601041) at 1/2,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 112 kDa
Observed band size: 140/110/75 kDa
Exposure time: 1 minute;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601041) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human seminoma tissue with Mouse anti-Sall4 antibody (HA601041) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601041) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"