iFluor™ 647 Conjugated SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44]
Catalog# HA720173F
iFluor™ 647 Conjugated SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44]
-
IF-Tissue
-
FC
-
Human
-
Mouse
-
Rat
概述
产品名称
iFluor™ 647 Conjugated SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human SATB2 238-287 / 733.
种属反应性
Human, Mouse, Rat
验证应用
IF-Tissue, FC
分子量
Predicted band size: 83 kDa
阳性对照
Human colon carcinoma tissue, THP-1.
偶联
iFluor™ 647
克隆号
JM52-44
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IF-Tissue
-
1:200
-
FC
-
1:500-1:1,000
靶点
功能
SATB2 (Special AT-rich sequence-binding protein 2) is a nuclear matrix protein that influences craniofacial formation mechanisms, such as jaw and palate development, and is part of a transcriptional network regulating skeletal development and osteoblast differentiation. Highly expressed in adult and fetal brain, SATB2 contains two CUT DNA-binding domains and one homeobox domain and is closely related to SATB1, a transcriptional repressor. SATB2 is thought to bind to matrix-attachment regions (MARs) and regulate MAR-dependent transcription of various genes, including HoxA2 and ATF4 (CREB-2), involved in skeletal development. Functioning as both a transcriptional activator and repressor, SATB2 can also act as a protein scaffold that can enhance the activity of other DNA-binding proteins. Defects in the gene encoding SATB2 are the cause of cleft palate manifested in conjunction with severe mental retardation.
背景文献
1. Mao H et al. Haploinsufficiency for Core Exon Junction Complex Components Disrupts Embryonic Neurogenesis and Causes p53-Mediated Microcephaly. PLoS Genet 12:e1006282 (2016).
2. Jaitner C et al. Satb2 determines miRNA expression and long-term memory in the adult central nervous system. Elife 5.pii: e17361 (2016).
序列相似性
Belongs to the CUT homeobox family.
组织特异性
High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
翻译后修饰
Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
亚细胞定位
Nucleus matrix.
UNIPROT
别名
DNA binding protein SATB2 antibody
DNA-binding protein SATB2 antibody
FLJ21474 antibody
FLJ32076 antibody
GLSS antibody
KIAA1034 antibody
MGC119474 antibody
MGC119477 antibody
SATB family member 2 antibody
SATB homeobox 2 antibody
展开图片
-
Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling SATB2 (HA720173F).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody SATB2 (HA720173F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. -
Flow cytometric analysis of THP-1 cells labeling SATB2.
Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with SATB2 (HA720173F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"