Palladin Recombinant Rabbit Monoclonal Antibody [JE66-56]
Catalog# HA721440
Palladin Recombinant Rabbit Monoclonal Antibody [JE66-56]
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WB
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Human
概述
产品名称
Palladin Recombinant Rabbit Monoclonal Antibody [JE66-56]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Palladin aa 1-100 / 1,383.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 151 kDa
阳性对照
HeLa cell lysate, SiHa cell lysate, A431 cell lysate, SW480 cell lysate, 293T cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, MCF7 cell lysate.
偶联
unconjugated
克隆号
JE66-56
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
靶点
功能
Palladin is a protein that in humans is encoded by the PALLD gene. Palladin is a component of actin-containing microfilaments that control cell shape, adhesion, and contraction. It is a part of the myotilin-myopalladin-palladin family and may play an important role in modulating the actin cytoskeleton. Palladin, in contrast to myotilin and myopalladin, which are expressed only in striated muscle, is expressed ubiquitously in cells of mesenchymal origin. Palladin's precise biological role is poorly understood, but it has been shown to play a role in cytoskeletal organization, embryonic development, cell motility, scar formation in the skin, and nerve cell development.
背景文献
1. Mayer O et al. Cytoskeletal Protein Palladin in Adult Gliomas Predicts Disease Incidence, Progression, and Prognosis. Cancers (Basel). 2022 Oct
2. Shu X et al. Palladin promotes cancer stem cell-like properties in lung cancer by activating Wnt/Β-Catenin signaling. Cancer Med. 2023 Feb
亚细胞定位
Cytoplasm, cytoskeleton, Cytoplasm, myofibril, sarcomere, Z line.
UNIPROT
别名
CGI 151 antibody
CGI151 antibody
KIAA0992 antibody
MYN antibody
Myoneurin antibody
pa antibody
Palladin cytoskeletal associated protein antibody
Palld antibody
PNCA1 antibody
Sarcoma antigen NY SAR 77 antibody
展开图片
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Western blot analysis of Palladin on different lysates with Rabbit anti-Palladin antibody (HA721440) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15µg/Lane)
Lane 2: SiHa cell lysate (10µg/Lane)
Lane 3: A431 cell lysate (15 µg/Lane)
Lane 4: SW480 cell lysate (30 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 8: MDA-MB-468 cell lysate (20 µg/Lane)
Lane 9: MCF7 cell lysate (20 µg/Lane)
Predicted band size: 151 kDa
Observed band size: 110/151 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721440) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"