• 概述

• 产品描述 O-linked N-acetylglucosamine (O-GlcNAc) transferase (also designated OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related; the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.
• 产品名称 iFluor™ 488 Conjugated Anti-OGT Recombinant Rabbit Monoclonal Antibody [JB44-39]
• 分子量 Predicted band size: 117 kDa
• 种属反应性 Human,Mouse,Rat
• 验证应用 IF-Cell
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within Human OGT aa 997-1,046 / 1,046.
• 偶联 iFluor™ 488

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Nucleus. Cytoplasm. Membrane.
• 数据链接 SwissProt: O15294 Human
  
• 其它名称
  • FLJ23071 antibody
  • GlcNAc transferase antibody
  • HRNT1 antibody
  • MGC22921 antibody
  • O GlcNAc antibody
  • O GlcNAc transferase p110 subunit antibody
  • O GlcNAc transferase subunit p110 antibody
  • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
  • O linked N acetylglucosamine (GlcNAc) transferase antibody
  • O linked N acetylglucosamine transferase 110 kDa subunit antibody
  • O-GlcNAc transferase subunit p110 antibody
  • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
  • ogt antibody
  • OGT1_HUMAN antibody
  • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
  • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
  • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
  • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
  • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
  • iFluor™488ConjugatedAnti-OGT
  more

• 应用

  IF-Cell: 1:75

• 

  Fig1: Immunocytochemistry analysis of Hela cells labeling OGT with Rabbit anti-OGT antibody (HA720156F) at 1/75 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-OGT antibody (HA720156F) at 1/75 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

• 背景文献

• 1. Yang X et al. Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: coupling protein O-GlcNAcylation to transcriptional repression. Cell 110:69-80 (2002).
  
  2. Yang X et al. Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance. Nature 451:964-969 (2008).

• Publishing research using iFluor™ 488 Conjugated Anti-OGT Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.