NQO1 Mouse Monoclonal Antibody [13A1]
Catalog# EM1801-21
NQO1 Mouse Monoclonal Antibody [13A1]
-
WB
-
Human
-
Rat
预测的种属支持售后
概述
产品名称
NQO1 Mouse Monoclonal Antibody [13A1]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human NQO1 aa 1-274 / 274.
种属反应性
Human (Predicted: Rat)
预测的种属支持售后
验证应用
WB
分子量
Predicted band size: 31 kDa
阳性对照
A549 cell lysate, SiHa cell lysate, HepG2 cell lysate, MCF7 cell lysate, HeLa cell lysate.
偶联
unconjugated
克隆号
13A1
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2b
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:2,000
靶点
功能
NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.
背景文献
1. Jaiswal A K et al. Human NAD(P)H:quinone oxidoreductase (NQO1) gene structure and induction by dioxin. Biochemistry 30:10647-10653 (1991).
2. Guryanova O A et al. Actin cytoskeleton remodeling by the alternatively spliced isoform of PDLIM4/RIL protein. J Biol Chem 286:26849-26859 (2011).
序列相似性
Belongs to the NAD(P)H dehydrogenase (quinone) family.
亚细胞定位
Cytoplasm.
别名
Azoreductase antibody
Cytochrome b 5 reductase antibody
DHQU antibody
DIA 4 antibody
DIA4 antibody
Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
Diaphorase (NADH/NADPH) antibody
Diaphorase 4 antibody
Dioxin inducible 1 antibody
DT diaphorase antibody
展开图片
-
☑ Relative expression (RE)
Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (EM1801-21) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: SiHa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: Saos-2 cell lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (EM1801-21) at 1/2,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si NQO1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"