Biotin Conjugated Mouse IL-17A Recombinant Rabbit Monoclonal Antibody [PS01-92] - Detector
概述
产品名称
Biotin Conjugated Mouse IL-17A Recombinant Rabbit Monoclonal Antibody [PS01-92] - Detector
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse IL17A aa 26-158 (HA210791).
种属反应性
Mouse
验证应用
ELISA(Det), ELISA
阳性对照
Recombinant Mouse IL-17A protein (HA210791).
偶联
Biotin
克隆号
PS01-92
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-91] to Mouse IL-17A antibody (Capture) (HA721606) and Recombinant Mouse IL-17A protein (HA210791) as the standard. The reference range value is 7.81-1,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
靶点
功能
Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance.
背景文献
1. Liang S.C., Long A.J., Bennett F., Whitters M.J., Karim R., Collins M., Goldman S.J., Dunussi-Joannopoulos K., Williams C.M., Wright J.F., Fouser L.A. An IL-17F/A heterodimer protein is produced by mouse Th17 cells and induces airway neutrophil recruitment. J. Immunol. 179:7791-7799 (2007).
2. Kuestner R.E., Taft D.W., Haran A., Brandt C.S., Brender T., Lum K., Harder B., Okada S., Ostrander C.D., Kreindler J.L., Aujla S.J. Identification of the IL-17 receptor related molecule IL-17RC as the receptor for IL-17F. J. Immunol. 179:5462-5473 (2007).
亚细胞定位
Secreted.
别名
CTLA 8 antibody
CTLA-8 antibody
CTLA8 antibody
cytotoxic T lymphocyte associated antigen 8 antibody
Cytotoxic T lymphocyte associated protein 8 antibody
Cytotoxic T lymphocyte associated serine esterase 8 antibody
Cytotoxic T-lymphocyte-associated antigen 8 antibody
IL 17 antibody
IL 17A antibody
IL-17 antibody
展开图片
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![Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies<br /><br />Capture: <a href="/products/HA721606" style="font-weight: bold;text-decoration: underline;">HA721606</a>, Mouse IL-17A Rabbit mAb [PS01-91]<br />Detector: <a href="/products/HA721607" style="font-weight: bold;text-decoration: underline;">HA721607</a>, Mouse IL-17A Rabbit mAb [PS01-92]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721606" style="font-weight: bold;text-decoration: underline;">HA721606</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17A protein (<a href="/products/HA210791" style="font-weight: bold;text-decoration: underline;">HA210791</a>) protein starting from 800 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA721607" style="font-weight: bold;text-decoration: underline;">HA721607</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721608_1.jpg?v=20250514073028)
Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies
Capture: HA721606, Mouse IL-17A Rabbit mAb [PS01-91]
Detector: HA721607, Mouse IL-17A Rabbit mAb [PS01-92]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721606) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17A protein (HA210791) protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721607, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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