MEMO1 Rabbit Polyclonal Antibody
Catalog# HA500024
MEMO1 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
MEMO1 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human MEMO1 aa 1-200.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 34 kDa
阳性对照
Mouse lung tissue lysate, mouse colon tissue lysate, mouse liver tissue, human skeletal muscle tissue, rat large intestine tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IHC-P
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1:100-1:500
靶点
功能
May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Is required for breast carcinoma cell migration.
背景文献
1. Marone R. et. al. Memo mediates ErbB2-driven cell motility. Nat. Cell Biol. 6:515-522(2004).
亚细胞定位
Cytosol, nucleus.
别名
C21orf19-like protein antibody
C2orf4 antibody
CGI 27 antibody
DKFZp434I0135 antibody
FLJ25031 antibody
HCV NS5A-transactivated protein 7 antibody
Hepatitis C virus NS5A-transactivated protein 7 antibody
Mediator of cell motility 1 antibody
Mediator of ErbB2-driven cell motility 1 antibody
MEMO antibody
展开图片
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Western blot analysis of MEMO1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500024, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse lung tissue lysate
Lane 2: Mouse colon tissue lysate -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MEMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500024, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-MEMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500024, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MEMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500024, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"