• 概述

• 产品描述 May control cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Mediator of ERBB2 signaling. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Is required for breast carcinoma cell migration.
• 产品名称 Anti-MEMO1 Rabbit Polyclonal Antibody
• 分子量 Predicted band size: 34 kDa.
• 种属反应性 Human,Mouse,Rat
• 验证应用 WB,IHC-P
• 抗体类型 兔多抗
• 免疫原 Recombinant protein within human MEMO1 aa 1-200.
• 偶联 Non-conjugated
• RRID AB_3071124

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Immunogen affinity purified.
• 亚细胞定位 Cytosol, nucleus.
• 数据链接 SwissProt: Q9Y316 Human
  SwissProt: Q91VH6 Mouse
  SwissProt: Q4QQR9 Rat
  
• 其它名称
  • C21orf19-like protein antibody
  • C2orf4 antibody
  • CGI 27 antibody
  • DKFZp434I0135 antibody
  • FLJ25031 antibody
  • HCV NS5A-transactivated protein 7 antibody
  • Hepatitis C virus NS5A-transactivated protein 7 antibody
  • Mediator of cell motility 1 antibody
  • Mediator of ErbB2-driven cell motility 1 antibody
  • MEMO antibody
  • Memo-1 antibody
  • memo1 antibody
  • MEMO1_HUMAN antibody
  • NS5ATP7 antibody
  • Protein MEMO1 antibody
  more

• 应用

  WB: 1:1,000-1:2,000
  IHC-P: 1:100-1:500

• 

  Fig1: Western blot analysis of MEMO1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500024, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Mouse lung tissue lysate
  Lane 2: Mouse colon tissue lysate

  

  Fig2: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MEMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500024, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-MEMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500024, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MEMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500024, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• 背景文献

• 1. Marone R. et. al. Memo mediates ErbB2-driven cell motility. Nat. Cell Biol. 6:515-522(2004).

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.