Kallikrein 5 Recombinant Rabbit Monoclonal Antibody [JE54-94]
Catalog# ET7110-90
Kallikrein 5 Recombinant Rabbit Monoclonal Antibody [JE54-94]
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WB
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IF-Cell
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FC
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Human
概述
产品名称
Kallikrein 5 Recombinant Rabbit Monoclonal Antibody [JE54-94]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Kallikrein 5 aa 161-293 / 293.
种属反应性
Human
验证应用
WB, IF-Cell, FC
分子量
Predicted band size: 32 kDa
阳性对照
MCF-7 cell lysate, SK-Br-3 cell lysate, MCF-7, A431.
偶联
unconjugated
克隆号
JE54-94
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:100
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FC
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1:50-1:100
靶点
功能
Kallikrein-related peptidase 5 (KLK5), formerly known as stratum corneum tryptic enzyme (SCTE), is a serine protease expressed in the epidermis. In humans it is encoded by the KLK5 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. Alternative splicing results in multiple transcript variants encoding the same protein. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
背景文献
1. Kasparek P. et. al. KLK5 and KLK7 Ablation Fully Rescues Lethality of Netherton Syndrome-Like Phenotype. PLoS Genet. 2017 Jan.
2. Pampalakis G. et. al. KLK5, a novel potential suppressor of vaginal carcinogenesis. Biol Chem. 2018 Sep.
序列相似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
组织特异性
Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.
亚细胞定位
Secreted.
UNIPROT
别名
HK5 antibody
Kallikrein 5 antibody
Kallikrein L2 antibody
Kallikrein like 2 antibody
Kallikrein like protein 2 antibody
Kallikrein related peptidase 5 antibody
Kallikrein-5 antibody
Kallikrein-like protein 2 antibody
Kallikrein5 antibody
KLK 5 antibody
展开图片
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Western blot analysis of Kallikrein 5 on different lysates with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/500 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: SK-Br-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Kallikrein 5 antibody (ET7110-90) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of Kallikrein 5 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"