• 概述

• 产品说明 The HSP/Chaperone Sampler Kit provides an economical means to investigate protein folding within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments with each antibody.

• 产品

• 产品包括	数量	应用	反应性	MW(kDa)
  HSPA14[ET1612-93]	20μl	WB,IF-Cell,IF-Tissue,IHC-P,IP	Human,Mouse,Rat	Predicted band size: 55 kDa
  Hsp70[ET1601-11]	20μl	WB,IF-Cell,IF-Tissue,IHC-P,FC	Human,Mouse,Rat	Predicted band size: 70 kDa
  GRP78[ER1706-50]	20μl	WB,IF-Cell,IHC-P,FC	Human,Mouse,Rat	Predicted band size: 72 kDa
  HSP40[EM2001-05]	20μl	WB	Human,Mouse,Rat	38 KDa
  Hsp90 beta[ET1605-56]	20μl	WB,IF-Cell,IF-Tissue,IHC-P,IP,FC	Human,Mouse,Rat	Predicted band size: 83 kDa
  Hsp90 alpha[ET1605-57]	20μl	WB,IF-Cell,IF-Tissue,IHC-P	Human,Mouse,Monkey,Rat	85 kDa
  Calmegin[ET7111-36]	20μl	WB,IHC-P,FC	Human,Mouse,Rat	70 kDa
  P4HB[ET7110-92]	20μl	WB,IF-Cell,IHC-P,FC	Human,Mouse,Rat	57 kDa
  HRP Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH[HA1031]	100μl	IP,ELISA,IHC-P,WB	Rabbit
  HRP Conjugated Goat anti-Mouse IgG[HA1006]	100μl	WB,ELISA,IHC-P	Mouse

• 

  Fig1: Western blot analysis of Hsp70 on different lysates with Rabbit anti-Hsp70 antibody (ET1601-11) at 1/2,000 dilution.
  
  Lane 1: HeLa cell lysate
  Lane 2: A549 cell lysate
  Lane 3: MCF7 cell lysate
  Lane 4: HCT 116 cell lysate
  Lane 5: Mouse brain tissue lysate
  Lane 6: Mouse testis tissue lysate
  Lane 7: Rat testis tissue lysate
  
  Lysates/proteins at 15 µg/Lane.
  
  Predicted band size: 70 kDa
  Observed band size: 70 kDa
  
  Exposure time: Lane 1-4: 43 seconds; Lane 5-7: 2 minutes;
  
  4-20% SDS-PAGE gel.
  
  Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-11) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Sample:
  Hela(Human) Cell Lysate at 30 ug
  Primary: Anti- HSP40 (EM2001-05) at 1/1000 dilution
  Secondary: Goat Anti-Mouse IgG at 1/20000 dilution
  Predicted band size: 38 kD
  Observed band size: 38 kD

• 背景

• HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress . HSP70 and HSP90 interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP-dependent manner.
  HSP40 family proteins bind unfolded proteins and prevent their aggregation, and deliver unfolded protiens to HSP70. HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria. Calenxin is a calcium-binding protein embedded in the ER membrane that retains newly synthesized glycoproteins inside the ER to ensure proper folding and quality control.Heat shock gene transcription is regulated by a familly of heat shock factors (HSFs), transcriptional activators that bind to heat shock response elements (HSEs) located upstream of all heat shock genes. During attenuation from the heat shock response, HSF1 is repressed by direct binding of HSP70, HSP40/Hdj-1 and HSF binding protein 1 (HSBP1).

• 背景文献

• 1. Nollen, E.A. and Morimoto, R.I. (2002) J. Cell Sci. 115, 2809-2816.
  
  2. Fan, C.Y. et al. (2003) Cell Stress Chaperones 8, 309-316.
  
  3. Jindal, S. et al. (1989) Mol Cell Biol 9, 2279-83.
  
  4. Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
  
  5. Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
  
  6. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
  
  7. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.
  
  8. Bergeron, J.J. et al. (1994) Trends Biochem. Sci. 19, 124-128.
  
  9. Williams, D.B. (2006) J. Cell Sci. 119, 615-623.
  
  10. Kohno, K. et al. (1993) Mol. Cell. Biol. 13, 877-890.
  
  11. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep. 6, 28-32.
  
  12. Morimoto, R.I. (1998) Genes Dev. 12, 3788-3796.
  
  13. Satyal, S.H. et al. (1998) Genes Dev. 12, 1962-1974.

• 数据库链接

• SwissProt: Q0VDF9 Human
  SwissProt: Q99M31 Mouse
  SwissProt: Q6AYB4 Rat
  SwissProt: P0DMV9 Human
  SwissProt: P0DMV8 Human
  SwissProt: P17879 Mouse
  SwissProt: Q61696 Mouse
  SwissProt: Q07439 Rat
  SwissProt: P11021 Human
  SwissProt: P20029 Mouse
  SwissProt: P06761 Rat
  SwissProt: P25685 Human
  SwissProt: P08238 Human
  SwissProt: P11499 Mouse
  SwissProt: P34058 Rat
  SwissProt: P07900 Human
  SwissProt: P07901 Mouse
  SwissProt: P82995 Rat
  SwissProt: O14967 Human
  SwissProt: P52194 Mouse
  SwissProt: P07237 Human
  SwissProt: P09103 Mouse
  SwissProt: P04785 Rat
  

• Publishing research using HSP/Chaperone Antibody Kit? Please let us know so that we can cite the reference in this datasheet.

Choose your product,application and species below,then click "Submit".

On the next page you can complete and submit your review.

Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.