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Anti-GAPDH Loading Control antibody [12D6]

小鼠单抗 | 货号: M1310-2


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价格:RMB   1200
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  • 概述

  • 产品描述 Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs and suppresses their translation.
  • 产品名称 Anti-GAPDH Loading Control antibody [12D6]
  • 分子量 36 kDa
  • 种属反应性 Human, Mouse, Rat, Zebrafish
  • 验证应用 WB, ICC
  • 抗体类型 小鼠单抗
  • 免疫原 Synthetic peptide within human GAPDH aa 180-220.
  • 偶联 Non-conjugated
  • 性能

  • 形态 Liquid
  • 浓度 2 mg/mL.
  • 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • 存储缓冲液 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • 亚型 IgG1
  • 纯化方式 Protein G affinity purified.
  • 亚细胞定位 Cytoplasm, nucleus.
  • 数据链接 SwissProt: P04406 Human
    SwissProt: P16858 Mouse
    SwissProt: P04797 Rat
  • 其它名称
    • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
    • aging associated gene 9 protein antibody
    • Aging-associated gene 9 protein antibody
  • 应用

    WB: 1:2000-1:5,000
    ICC: 1:200

  • 背景文献

  • 1. Cheng, Y., Hou, T., Ping, J., Chen, T., & Yin, B. (2018). LMO3 promotes hepatocellular carcinoma invasion, metastasis and anoikis inhibition by directly interacting with LATS1 and suppressing Hippo signaling. J Exp Clin Cancer Res, 37(1), 228.

    2. Liu, J., Wang, Y., Song, L., Zeng, L., Yi, W., Liu, T., . . . Cong, Y. S. (2017). A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1alpha stability. Nat Commun, 8, 14186.

    3. Jiang, Y., Tian, M., Lin, W., Wang, X., & Wang, X. (2018). Protein Kinase Serine/Threonine Kinase 24 Positively Regulates Interleukin 17-Induced Inflammation by Promoting IKK Complex Activation. Front Immunol, 9, 921.

    4. Bin, G., Jiarong, Z., Shihao, W., Xiuli, S., Cheng, X., Liangbiao, C., & Ming, Z. (2012). Aire promotes the self-renewal of embryonic stem cells through Lin28. Stem Cells Dev, 21(15), 2878-2890.

    5. Liu, J., Wang, Y., Song, L., et al. Cong, Y. S. (2017). A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1alpha stability. Nat Commun, 8, 14186. doi: 10.1038/ncomms14186

    6. Zhang, D., Zhao, Q., Sun, H., Yin, L., Wu, J., Xu, J., . . . Liang, C. (2016). Defective autophagy leads to the suppression of stem-like features of CD271(+) osteosarcoma cells. J Biomed Sci, 23(1), 82. doi: 10.1186/s12929-016-0297-5
  • Publishing research using Anti-GAPDH Loading Control antibody? Please let us know so that we can cite the reference in this datasheet.

  • PMID:
    35036955
    Optimized protocol to detect protein UFMylation in cells and in vitro via immunoblotting Author: Zhou, J., Liang, Q., Dong, M., Ma, X., Jin, Y., Guan, D., Liu, J., Wang, M., & Cong, Y. S. Published: 2022 Application: WB Species: Human Journal: STAR Protocols
  • PMID:
    32468020
    Upregulation of miRNA-301a-3p promotes tumor progression in gastric cancer by suppressing NKRF and activating NF-κB signaling Author: Xujun He Published: 2020 Application: WB Species: human Journal: INTERNATIONAL JOURNAL OF ONCOLOGY IF: 3.899
  • PMID:
    32236608
    CD44 inhibition attenuates EGFR signaling and enhances cisplatin sensitivity in human EGFR wild-type non-small-cell lung cancer cells Author: Dandan Yuan;Mingxing Li Published: 2020 Application: WB,IF Species: human Journal: Int J Mol Med. IF: 2.928
  • PMID:
    32903879
    Integrative Analysis of the IQ Motif-Containing GTPase-Activating Protein Family Indicates That the IQGAP3-PIK3C2B Axis Promotes Invasion in Colon Cancer Author: Yuping Zhu; Xujun He Published: 2020 Application: WB Species: human Journal: OncoTargets and Therapy IF: 3.337
  • PMID:
    30989650
    Identification of the potential key genes for adipogenesis from human mesenchymal stem cells by RNA‐Seq Author: Xia Yi,Xiaoyuan Xu,and Weidong Li Published: 2019 Application: WB Species: hMSCs Journal: J Cell Physiol IF: 4.522
  • PMID:
    28128204
    A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1a stability Author: Zhenyu Ju & Yu-Sheng Cong Published: 2017 Application: WB Species: HEK293T,MCF7 and HepG2 cells Journal: Nat Commun IF: 11.3

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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