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概述
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产品描述
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
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产品名称
Anti-GAPDH Recombinant Rabbit Monoclonal Antibody
[SA30-01]
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分子量
Predicted band size: 36 kDa
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种属反应性
Human,Mouse,Rat,Chicken, Zebrafish
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验证应用
WB,IF-Cell,IF-Tissue,IHC-P,FC,IP
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抗体类型
重组兔单抗
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免疫原
Recombinant protein within mouse GAPDH aa 94-333 / 333.
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偶联
Non-conjugated
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性能
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形态
Liquid
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浓度
1 mg/mL.
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存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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亚型
IgG
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纯化方式
Protein A affinity purified.
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亚细胞定位
Cytoplasm, Nucleus, Membrane
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数据链接
SwissProt: P04406 Human
SwissProt: P16858 Mouse
SwissProt: P04797 Rat
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其它名称
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
- BARS-38 antibody
- cb609 antibody
- EC 1.2.1.12 antibody
- Epididymis secretory sperm binding protein Li 162eP antibody
- G3P_HUMAN antibody
- G3PD antibody
- G3PDH antibody
- GAPD antibody
- GAPDH antibody
- Glyceraldehyde 3 phosphate dehydrogenase antibody
- Glyceraldehyde-3-phosphate dehydrogenase antibody
- HEL-S-162eP antibody
- KNC-NDS6 antibody
- MGC102544 antibody
- MGC102546 antibody
- MGC103190 antibody
- MGC103191 antibody
- MGC105239 antibody
- MGC127711 antibody
- MGC88685 antibody
- OCAS, p38 component antibody
- OCT1 coactivator in S phase, 38-KD component antibody
- peptidyl cysteine S nitrosylase GAPDH antibody
- Peptidyl-cysteine S-nitrosylase GAPDH antibody
- wu:fb33a10 antibody
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应用
WB: 1:5,000-1:640,000
IF-Cell: 1:50
IF-Tissue: 1:50
IHC-P: 1:50
FC: 1:50
IP: Use at an assay dependent concentration.
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Fig1: Western blot analysis of GAPDH on Hela cell lysates with Rabbit anti-GAPDH antibody (ET1601-4).
Hela cell lysates at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4, 1/5,000) was used in 5% NFDM/TBST at room temperature for 1 hour. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 45 mins at room temperature.
Positive control:
Lane 1: PC-3 cell lysate, 10 µg/Lane
Lane 2: Mouse colon tissue lysate, 20 µg/Lane
Lane 3: SH-SY5Y cell lysate, 10 µg/Lane
Lane 4: PC-3 cell lysate, 10 µg/Lane
Lane 5: NIH/3T3 cell lysate, 10 µg/Lane
Lane 6: SK-Br-3 cell lysate, 10 µg/Lane
Lane 7: Rat brain tissue lysate, 20 µg/Lane
Fig3: Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4, 1/10,000) was used in 5% NFDM/TBST at room temperature for 1 hour. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 45 mins at room temperature.
Positive control:
Lane 1: Rat liver tissue lysate, 20 µg/Lane
Lane 2: Rat lung tissue lysate, 20 µg/Lane
Lane 3: Rat lung tissue lysate, 20 µg/Lane
Lane 4: Rat heart tissue lysate, 20 µg/Lane
Lane 5: Rat cerebellum tissue lysate, 20 µg/Lane
Lane 6: Rat skeletal muscle tissue lysate, 20 µg/Lane
Lane 7: Rat spleen tissue lysate, 20 µg/Lane
Lane 8: Rat small intestine tissue lysate, 20 µg/Lane
Fig4: Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (ET1601-4) at 1/80,000 dilution.
Cell lysates at 10 µg/Lane, tissue lysates at 20 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4) at 1/80,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Fig5: Western blot analysis of GAPDH on DF-1 cell lysates with Rabbit anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution.
Cell lysates at 15 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 1 second;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 2 hour at room temperature. The primary antibody (ET1601-4) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 ℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 2 hour at room temperature.
Fig6: ICC staining of GAPDH in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Fig7: ICC staining of GAPDH in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GAPDH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-4, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-GAPDH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-4, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig10: Flow cytometric analysis of GAPDH was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1601-4, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
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背景文献
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1. "High-resolution structure of human D-glyceraldehyde-3-phosphate dehydrogenase."Jenkins J.L., Tanner J.J.Acta Crystallogr. D 62:290-301(2006)
2. "Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase."Ismail S.A., Park H.W.Acta Crystallogr. D 61:1508-1513(2005)
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Publishing research
using Anti-GAPDH Recombinant Rabbit Monoclonal Antibody? Please let us know so
that we can cite the reference in this datasheet.
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PMID:
36781743
Alpha-Asarone Ameliorates Neurological Dysfunction of Subarachnoid Hemorrhagic Rats in Both Acute and Recovery Phases via Regulating the CaMKII-Dependent Pathways
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PMID:
36890454
Cyclic tensile force modifies calvarial osteoblast function via the interplay between ERK1/2 and STAT3
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PMID:
37261388
The Mechanosensitive Piezo1 Channel Mediates Mechanochemical Transmission in Myopic Eyes
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PMID:
36609254
Analysis of the expression and prognostic significance of DDK complex in Hepatocarcinoma
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PMID:
36815967
Roles of PADI4 in the expression of cytokines involved in inflammation and adhesion in differentiated NB4 cells treated with ATRA
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PMID:
36814859
Characterization of early myocardial inflammation in ischemia-reperfusion injury
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PMID:
36604795
Transcription factor ETS proto-oncogene 1 contributes to neuropathic pain by regulating histone deacetylase 1 in primary afferent neurons
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PMID:
36776388
DSS-induced colitis activates the kynurenine pathway in serum and brain by affecting IDO-1 and gut microbiota
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PMID:
37434162
Identification of a novel role for TL1A/DR3 deficiency in acute respiratory distress syndrome that exacerbates alveolar epithelial disruption
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PMID:
36597142
(Pro)renin receptor promotes colorectal cancer progression through inhibiting the NEDD4L-mediated Wnt3 ubiquitination and modulating gut microbiota
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PMID:
37033323
LAMP5 may promote MM progression by activating p38
Author: Chen, Y., & Ma, T. Published: 2023 Application: WB Species: Human Journal: Pathol Oncol Res IF: 2.874
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PMID:
36983022
dCas9-Based PDGFR-β Activation ADSCs Accelerate Wound Healing in Diabetic Mice through Angiogenesis and ECM Remodeling
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PMID:
35957979
Vitamin D Receptor Genetic Polymorphisms Associate With a Decreased Susceptibility to Extremity Osteomyelitis Partly by Inhibiting Macrophage Apoptosis Through Inhibition of Excessive ROS Production via VDR-Bmi1 Signaling
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PMID:
35602963
Glycyrrhetinic acid restricts mitochondrial energy metabolism by targeting SHMT2
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PMID:
36387145
Shikonin induces ferroptosis in multiple myeloma via GOT1-mediated ferritinophagy
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PMID:
34873906
Targeted Inhibition of Tumor Inflammation and Tumor-Platelet Crosstalk by Nanoparticle-Mediated Drug Delivery Mitigates Cancer Metastasis
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PMID:
35529046
A sustained release of BMP2 in urine-derived stem cells enhances the osteogenic differentiation and the potential of bone regeneration
Author: Wu, S., Chen, Z., Yu, X., Duan, X., Chen, J., Liu, G., Gong, M., Xing, F., Sun, J., Huang, S., & Xiang, Z. Published: 2022 Application: WB Species: Human Journal: Regen Biomater IF: 6.353
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PMID:
36310381
Overexpression of adrenomedullin (ADM) alleviates the senescence of human dental pulp stem cells by regulating the miR-152/CCNA2 pathway
Author: Dou, W., Xie, J., Chen, J., Zhou, J., Xu, Z., Wang, Z., & Zhu, Q. Published: 2022 Application: WB Species: Human Journal: Cell Cycle. IF: 5.173
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PMID:
35359966
TL1A/DR3 Axis, A Key Target of TNF-a, Augments the Epithelial–Mesenchymal Transformation of Epithelial Cells in OVA-Induced Asthma
Author: Zhang, D., Yang, H., Dong, X. L., Zhang, J. T., Liu, X. F., Pan, Y., Zhang, J., Xu, J. W., Wang, Z. H., Cui, W. J., & Dong, L. Published: 2022 Application: WB Species: Human Journal: Front Immunol IF: 7.561
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PMID:
36126794
NORAD promotes multiple myeloma cell progression via BMP6/P-ERK1/2 axis
Author: Ma, T., Chen, Y., Yi, Z. G., Liu, J., Li, Y. H., Bai, J., Tie, W. T., Huang, M., Zhu, X. F., Wang, J., Du, J., Zuo, X. Q., Li, Q., Lin, F. L., Tang, L., Guo, J., Xiao, H. W., Lei, Q., Ma, X. L., Li, L. J., … Zhang, L. S. Published: 2022 Journal: Cell Signal. IF: 4.850
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PMID:
35913215
Negative Regulatory Role of the Spring Viremia of Carp Virus Matrix Protein in the Host Interferon Response by Targeting the MAVS/TRAF3 Signaling Axis
Author: Wang, Y. Y., Chen, Y. L., Ji, J. F., Fan, D. D., Lin, A. F., Xiang, L. X., & Shao, J. Z. Published: 2022 Application: WB Species: Zebrafish Journal: J Virol IF: 6.549
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PMID:
36104705
miR-210-3p suppresses osteogenic differentiation of MC3T3-E1 by targeting brain derived neurotrophic factor (BDNF)
Author: Deng, L., Lai, S., Fan, L., Li, X., Huang, H., & Mu, Y. Published: 2022 Application: WB Species: Mouse Journal: J Orthop Surg Res. IF: 2.677
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PMID:
36076940
miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts
Author: Zhang, G., Zhang, X., Zhou, K., Ling, X., Zhang, J., Wu, P., Zhang, T., Xie, K., & Dai, G. Published: 2022 Application: WB Species: Chicken Journal: Int J Mol Sci. IF: 6.208
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PMID:
36611865
Effect of SNHG11/miR-7-5p/PLCB1 Axis on Acute Pancreatitis through Inhibiting p38MAPK Pathway
Author: Song, T. J., Ke, J., Chen, F., Zhang, J. Y., Zhang, C., & Chen, H. Y. Published: 2022 Application: WB Species: Rat Journal: Cells IF: 7.666
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PMID:
36124234
Integrative analysis reveals histone demethylase LSD1 promotes RNA polymerase II pausing
Author: Kim, H. J., Li, P., Kim, T., Oldfield, A. J., Zheng, X., & Yang, P. Published: 2022 Application: WB Journal: iScience. IF: 6.107
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PMID:
35652200
A Temporal PROTAC Cocktail-Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells
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PMID:
35148462
SGK1‐targeted TRPV1 regulates bladder smooth muscle cell proliferation due to BOO in mice via NFAT2
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PMID:
36497433
Comprehensive Pan-Cancer Analyses of Immunogenic Cell Death as a Biomarker in Predicting Prognosis and Therapeutic Response
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PMID:
35296033
The Food Additive β-Caryophyllene Exerts Its Neuroprotective Effects Through the JAK2-STAT3-BACE1 Pathway
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PMID:
35950373
Downregulation of fibulin-4 inhibits autophagy and promotes the sensitivity of esophageal squamous cell carcinoma cells to apatinib by activating the Akt-mTOR signaling pathway
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PMID:
36147444
Effect of Kaempferol on the Biological Behavior of Human Colon Cancer via Regulating MMP1, MMP2, and MMP9
Author: Zhang, X., Fan, J., Guo, F., Huang, H., Xue, J., Wu, X., & Li, T. Published: 2022 Application: WB Species: Human Journal: J Oncol. IF: 4.501
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PMID:
36140742
MYOZ1 Gene Promotes Muscle Growth and Development in Meat Ducks
Author: Zhou, T., Wu, Y., Bi, Y., Bai, H., Jiang, Y., Chen, G., Chang, G., & Wang, Z. Published: 2022 Application: WB Journal: Genes (Basel). IF: 4.141
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PMID:
36338340
Dynamin-Related Protein 1 Is Involved in Mitochondrial Damage, Defective Mitophagy, and NLRP3 Inflammasome Activation Induced by MSU Crystals
Author: Jiang, H., Chen, F., Song, D., Zhou, X., Ren, L., & Zeng, M. Published: 2022 Application: WB Species: Mouse Journal: Oxid Med Cell Longev. IF: 7.310
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PMID:
34907172
Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling
Author: Yin, P., Li, D., Zhao, Q., Cai, M., Wu, Z., Shi, Y., & Su, L. Published: 2021 Application: WB Journal: Cell Death Discov IF: 5.24
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PMID:
34001083
Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis. Microbial cell factories, 20(1), 102.
Author: Zhang, F., Wang, B., Liu, S., Chen, Y., Lin, Y., Liu, Z., Zhang, X., & Yu, B. Published: 2021 Application: WB Species: Mouse Journal: Microb Cell Fact
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PMID:
34157317
Cordycepin inhibits inflammatory responses through suppression of ERK activation in zebrafish. Developmental and comparative immunology, 124, 104178. Advance online publication.
Author: Liu, W., Zhang, L., Sun, S., Tang, L. S., He, S. M., Chen, A. Q., Yao, L. N., & Ren, D. L. Published: 2021 Application: WB Species: Zebrafish Journal: Dev Comp Immunol
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PMID:
34764672
Syndecan-1 Shedding by Matrix Metalloproteinase-9 Signaling Regulates Alveolar Epithelial Tight Junction in Lipopolysaccharide-Induced Early Acute Lung Injury
Author: Zhang, D., Zhang, J. T., Pan, Y., Liu, X. F., Xu, J. W., Cui, W. J., Qiao, X. R., & Dong, L. Published: 2021 Application: WB Species: Mouse Journal: J Inflamm Res IF: 6.922
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PMID:
34532112
Expression, clinical significance and correlation of RUNX3 and HER2 in colorectal cancer
Author: Wu, Y., Xue, J., Li, Y., Wu, X., Qu, M., Xu, D., & Shi, Y. Published: 2021 Application: WB Journal: J Gastrointest Oncol IF: 2.892
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PMID:
32319638
MicroRNA-125a-mediated regulation of the mevalonate signaling pathway contributes to high glucose-induced proliferation and migration of vascular smooth muscle cells
Author: Shen-jiang Hu Published: 2020 Application: WB Species: human Journal: Molecular Medicine REPORTS IF: 1.851
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PMID:
33384997
Antenatal Corticosteroid Therapy Attenuates Angiogenesis Through Inhibiting Osteoclastogenesis in Young Mice
Author: Bin Yu;Xianrong Zhang Published: 2020 Application: WB Species: mice Journal: Front Cell Dev Biol. IF: 5.201
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PMID:
33184293
Pharmacological targeting of MCL-1 promotes mitophagy and improves disease pathologies in an Alzheimer’s disease mouse model
Author: Jie Wu ;Ayaz Najafov;Hongguang Xia Published: 2020 Application: WB,COIP Species: human Journal: NATURE COMMUNICATIONS IF: 12.121
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PMID:
32943604
Tumor-derived HMGB1 induces CD62Ldim neutrophil polarization and promotes lung metastasis in triple-negative breast cancer
Author: Jian Huang Published: 2020 Application: WB Species: mice Journal: Oncogenesis. IF: 6.119
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PMID:
31298955
The role of EGFR signaling in age-related osteoporosis in mouse cortical bone
Author: Liu, G., Xie, Y., Su, J., Qin, H., Wu, H., Li, K., Yu, B., & Zhang, X. Published: 2019 Application: WB Species: Mouse Journal: FASEB J. IF: 5.192
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PMID:
31638230
Theaflavin attenuates cerebral ischemia/reperfusion injury by abolishing miRNA-128-3p-mediated Nrf2 inhibition and reducing oxidative stress
Author: Li Fei,Jianhong Zhu Published: 2019 Application: WB Species: rat Journal: Molecular Medicine REPORTS IF: 1.851
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