Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function.
iFluor™ 488 Conjugated Anti-E-Cadherin Recombinant Rabbit Monoclonal Antibody
Predicted band size: 97 kDa
Synthetic peptide within Human E-Cadherin aa 591-640 / 882.
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Protein A affinity purified.
Endosome, Cell membrane, trans-Golgi network, adherens junction.
SwissProt: P12830 Human
- Arc 1 antibody
- CADH1_HUMAN antibody
- Cadherin 1 antibody
- cadherin 1 type 1 E-cadherin antibody
- Cadherin1 antibody
- CAM 120/80 antibody
- CD 324 antibody
- CD324 antibody
- CD324 antigen antibody
- cdh1 antibody
- CDHE antibody
- E-Cad/CTF3 antibody
- E Cadherin antibody
- E-cadherin antibody
- ECAD antibody
- Epithelial cadherin antibody
- epithelial calcium dependant adhesion protein antibody
- LCAM antibody
- Liver cell adhesion molecule antibody
- UVO antibody
- Uvomorulin antibody
Fig1: Immunocytochemistry analysis of MCF-7 cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
Fig2: Flow cytometric analysis of A431 cells labeling E-Cadherin.
Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Fig3: Flow cytometric analysis of MCF-7 cells labeling E-Cadherin.
Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 10ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 10ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
1. Su B et al. Diallyl disulfide suppresses epithelial-mesenchymal transition, invasion and proliferation by downregulation of LIMK1 in gastric cancer. Oncotarget 7:10498-512 (2016).
2. Schmidt TP et al. Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA. Sci Rep 6:23264 (2016).
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