• 概述

• 产品描述 Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function.
• 产品名称 iFluor™ 488 Conjugated Anti-E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287]
• 分子量 Predicted band size: 97 kDa
• 种属反应性 Human
• 验证应用 IF-Cell,FC
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within Human E-Cadherin aa 591-640 / 882.
• 偶联 iFluor™ 488

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Endosome, Cell membrane, trans-Golgi network, adherens junction.
• 数据链接 SwissProt: P12830 Human
  
• 其它名称
  • Arc 1 antibody
  • CADH1_HUMAN antibody
  • Cadherin 1 antibody
  • cadherin 1 type 1 E-cadherin antibody
  • Cadherin1 antibody
  • CAM 120/80 antibody
  • CD 324 antibody
  • CD324 antibody
  • CD324 antigen antibody
  • cdh1 antibody
  • CDHE antibody
  • E-Cad/CTF3 antibody
  • E Cadherin antibody
  • E-cadherin antibody
  • ECAD antibody
  • Epithelial cadherin antibody
  • epithelial calcium dependant adhesion protein antibody
  • LCAM antibody
  • Liver cell adhesion molecule antibody
  • UVO antibody
  • Uvomorulin antibody
  • iFluor™488ConjugatedAnti-E-Cadherin
  more

• 应用

  IF-Cell: 1:100
  FC: 1:50-1:1,000

• 

  Fig1: Immunocytochemistry analysis of MCF-7 cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

  

  Fig2: Flow cytometric analysis of A431 cells labeling E-Cadherin.
  
  Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  

  Fig3: Flow cytometric analysis of MCF-7 cells labeling E-Cadherin.
  
  Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 10ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 10ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• 背景文献

• 1. Su B et al. Diallyl disulfide suppresses epithelial-mesenchymal transition, invasion and proliferation by downregulation of LIMK1 in gastric cancer. Oncotarget 7:10498-512 (2016).
  
  2. Schmidt TP et al. Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA. Sci Rep 6:23264 (2016).

• Publishing research using iFluor™ 488 Conjugated Anti-E-Cadherin Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.