iFluor™ 594 Conjugated Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody [SA30-06]

Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 19.
Cells were fixed in 4% paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 10 minutes, and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with HA720115F at 1/50 dilution Rabbit monoclonal to Cytokeratin 19 (iFluor™ 594)(shown in red). DAPI was used as nuclear counterstain.

Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 19.
Cells were fixed in 100% methanol and permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. The cells were then incubated overnight at +4℃ with HA720115F at 1/50 dilution Rabbit monoclonal to Cytokeratin 19 (iFluor™ 594)(shown in red). DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 19 (HA720115F).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 19 (HA720115F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 19 (HA720115F).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 19 (HA720115F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Flow cytometric analysis of MCF-7 cells labeling Cytokeratin 19.

Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 19 (HA720115F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 19.

Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 19 (HA720115F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).