• 概述

• 产品描述 Clenbuterol is a sympathomimetic amine used by sufferers of breathing disorders as a decongestant and bronchodilator. People with chronic breathing disorders such as asthma use this as a bronchodilator to make breathing easier. Clenbuterol is pharmacological similarities to epinephrine and salbutamol, but its effects are more potent and longer-lasting as a stimulant and thermogenic drug. It is commonly used for smooth muscle-relaxant properties as a bronchodilator and tocolytic. Use over the recommended dose of about 120 μg can cause muscle tremors, headache, dizziness, and gastric irritation. Persons self-administering the drug for weight loss or to improve athletic performance have experienced nausea, vomiting, diaphoresis, palpitations, tachycardia, and myocardial infarction. Clenbuterol is not an ingredient of any therapeutic drug approved by the US Food and Drug Administration[citation needed] and is now banned for IOC-tested athletes. A common misconception about Clenbuterol is that it has anabolic properties, and can increase muscle mass when used in higher dosages. Use of the drug may be confirmed by detecting its presence in semen or urine. This antibody [PSH0-01] cross-reacts with albuterol and Ractopamine.
• 产品名称 Anti-Clenbuterol Recombinant Rabbit Monoclonal Antibody [PSH0-01]
• 验证应用 ELISA
• 抗体类型 重组兔单抗
• 免疫原 Clenbuterol-OVA
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 1mg/ml
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 PBS (pH7.4).
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 其它名称
  • Clenbuterol antibody

• 应用

  ELISA: 1:5,000-1:20,000

• 

  Fig1: Indirect ELISA analysis of Clenbuterol was performed by coating wells of a 96-well plate with 50 µl per well of Clenbuterol-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Clenbuterol monoclonal antibody serial diluted starting from a concentration of 20ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  

  Fig2: Competitive ELISA analysis of Clenbuterol / Salbutamol/ Ractopamine was performed by coating wells of a 96-well plate with 50 µl per well of Clenbuterol-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Clenbuterol monoclonal antibody at concentration of 1 µg/mL with serial diluted Clenbuterol/Salbutamol/Ractopamine starting from a concentration of 10ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1: 5,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

• 背景文献

• 1. Pluim BM, de Hon O, Staal JB, Limpens J, Kuipers H, Overbeek SE, et al. (January 2011). "β₂-Agonists and physical performance: a systematic review and meta-analysis of randomized controlled trials". Sports Medicine. 41 (1): 39–57.
  
  2. R. Baselt, Disposition of Toxic Drugs and Chemicals in Man, 8th edition, Biomedical Publications, Foster City, CA, 2008, pp. 325–326.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.