Clenbuterol Recombinant Rabbit Monoclonal Antibody [PSH0-01] - BSA and Azide free
Catalog# HA721229
Clenbuterol Recombinant Rabbit Monoclonal Antibody [PSH0-01] - BSA and Azide free
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ELISA
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Species independent
概述
产品名称
Clenbuterol Recombinant Rabbit Monoclonal Antibody [PSH0-01] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Clenbuterol-OVA
种属反应性
Species independent
验证应用
ELISA
偶联
unconjugated
克隆号
PSH0-01
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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ELISA
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1:5,000-1:20,000
靶点
功能
Clenbuterol is a sympathomimetic amine used by sufferers of breathing disorders as a decongestant and bronchodilator. People with chronic breathing disorders such as asthma use this as a bronchodilator to make breathing easier. Clenbuterol is pharmacological similarities to epinephrine and salbutamol, but its effects are more potent and longer-lasting as a stimulant and thermogenic drug. It is commonly used for smooth muscle-relaxant properties as a bronchodilator and tocolytic. Use over the recommended dose of about 120 μg can cause muscle tremors, headache, dizziness, and gastric irritation. Persons self-administering the drug for weight loss or to improve athletic performance have experienced nausea, vomiting, diaphoresis, palpitations, tachycardia, and myocardial infarction. Clenbuterol is not an ingredient of any therapeutic drug approved by the US Food and Drug Administration[citation needed] and is now banned for IOC-tested athletes. A common misconception about Clenbuterol is that it has anabolic properties, and can increase muscle mass when used in higher dosages. Use of the drug may be confirmed by detecting its presence in semen or urine. This antibody [PSH0-01] cross-reacts with albuterol and Ractopamine.
背景文献
1. Pluim BM, de Hon O, Staal JB, Limpens J, Kuipers H, Overbeek SE, et al. (January 2011). "β₂-Agonists and physical performance: a systematic review and meta-analysis of randomized controlled trials". Sports Medicine. 41 (1): 39–57.
2. R. Baselt, Disposition of Toxic Drugs and Chemicals in Man, 8th edition, Biomedical Publications, Foster City, CA, 2008, pp. 325–326.
别名
Clenbuterol antibody
图片
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Indirect ELISA analysis of Clenbuterol was performed by coating wells of a 96-well plate with 50 µl per well of Clenbuterol-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Clenbuterol monoclonal antibody serial diluted starting from a concentration of 20ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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Competitive ELISA analysis of Clenbuterol / Salbutamol/ Ractopamine was performed by coating wells of a 96-well plate with 50 µl per well of Clenbuterol-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Clenbuterol monoclonal antibody at concentration of 1 µg/mL with serial diluted Clenbuterol/Salbutamol/Ractopamine starting from a concentration of 10ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1: 5,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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