Fig1: Immunoprecipitating IgG and DYKDDDDK Tag (FLAG)(M1403-2) in recombinant protein with falg on N-terminal. 25ng of Immunoprecipitated protein incubated with primary antibody (1/1000) for over night at 4℃. For western blotting a IP Detection Reagent (NBI02H) (1/1000) was used to confirm successful immunoprecipation.
Lane1/2: Immunoprecipitating IgG in recombinant protein with falg on N-terminal
Lane3: Immunoprecipitating DYKDDDDK Tag (FLAG) (M1403-2) in recombinant protein with falg on N-terminal
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.Click here to view the general protocols