• Overview

• Storage Buffer PBS PH=7.5, 10mg/ml BSA, 100mM trehalose, 50% glycerol.
• Concentration 1 mg/mL.
• Recommended dilution WB: 1:1,000-1:10,000
• Stability and Storage Store at –20 ℃, protect from light.

• 

  Fig1: Immunoprecipitating IgG and DYKDDDDK Tag (FLAG)(M1403-2) in recombinant protein with falg on N-terminal. 25ng of Immunoprecipitated protein incubated with primary antibody (1/1000) for over night at 4℃. For western blotting a IP Detection Reagent (NBI02H) (1/1000) was used to confirm successful immunoprecipation.
  Lane1/2: Immunoprecipitating IgG in recombinant protein with falg on N-terminal
  Lane3: Immunoprecipitating DYKDDDDK Tag (FLAG) (M1403-2) in recombinant protein with falg on N-terminal

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.