HTF9C Recombinant Rabbit Monoclonal Antibody [JE55-60]
Rmb: 880 特惠 1500
Catalog# ET7111-14
HTF9C Recombinant Rabbit Monoclonal Antibody [JE55-60]
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WB
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IHC-P
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IF-Cell
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Human
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unconjugated
概述
产品名称
HTF9C Recombinant Rabbit Monoclonal Antibody [JE55-60]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant fragment within N-terminal Human HTF9C.
种属反应性
Human
验证应用
WB, IHC-P, IF-Cell
靶点分子量
Predicted band size: 69 kDa
阳性对照
293 cell lysate, Jurkat cell lysate, MCF-7 cell lysate, human liver carcinoma tissue, human thyroid tissue, human breast carcinoma tissue, 293.
偶联
unconjugated
克隆号
JE55-60
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:100
靶点
功能
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.
背景文献
1. Chang YH. et. al. TRMT2A is a novel cell cycle regulator that suppresses cell proliferation. Biochem Biophys Res Commun. 2019 Jan
2. Hicks DG. et. al. The expression of TRMT2A, a novel cell cycle regulated protein, identifies a subset of breast cancer patients with HER2 over-expression that are at an increased risk of recurrence. BMC Cancer. 2010 Mar
亚细胞定位
Nucleoplasm and Cytosol.(Predicted)
UNIPROT
别名
HpaII tiny fragments locus 9c protein antibody
HTF9C antibody
TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody
TRM2 tRNA methyltransferase 2 homolog A antibody
TRM2A_HUMAN antibody
TRMT2A antibody
tRNA (uracil-5-)-methyltransferase homolog A antibody
tRNA methyltransferase 2 homolog A antibody
tRNA methyltransferase 2, S. cerevisiae, homolog of, A antibody
图片
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Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunocytochemistry analysis of 293 cells labeling HTF9C with Rabbit anti-HTF9C antibody (ET7111-14) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HTF9C antibody (ET7111-14) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Western blot analysis of HTF9C on different lysates with Rabbit anti-HTF9C antibody (ET7111-14) at 1/2,000 dilution.
Lane 1: HEK-293(Human embryonic kidney cell) cell lysate
Lane 2: 293T(Human embryonic kidney cell) cell lysate+
Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 4: MCF-7 (Human breast cancer cell) cell lysate
Lane 5: HepG2 (Human hepatocellular carcinoma cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7111-14, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 69 kDa
Observed band size: 75 kDa
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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