• 概述

• 产品描述 Caspase-9 belongs to a family of caspases, cysteine-aspartic proteases involved in apoptosis and cytokine signalling. Apoptotic signals cause the release of cytochrome c from mitochondria and activation of apaf-1 (apoptosome), which then cleaves the pro-enzyme of caspase-9 into the active dimer form. Regulation of this enzyme occurs through phosphorylation by an allosteric inhibitor, inhibiting dimerization and inducing a conformational change. Correct caspase-9 function is required for apoptosis, leading to the normal development of the central nervous system. Caspase-9 has multiple additional cellular functions that are independent of its role in apoptosis. Nonapoptotic roles of caspase-9 include regulation of necroptosis, cellular differentiation, innate immune response, sensory neuron maturation, mitochondrial homeostasis, corticospinal circuit organization, and ischemic vascular injury.
• 产品名称 Anti-Caspase-9 Recombinant Rabbit Monoclonal Antibody [SC65-05]
• 分子量 Predicted band size: 46/30/17/37 kDa
• 种属反应性 Human,Mouse
• 验证应用 WB,IP
• 抗体类型 重组兔单抗
• 免疫原 Recombinant protein within Human Caspase-9 aa 95-335 / 416.
• 偶联 Non-conjugated
• RRID AB_2940743

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Apoptosome, cytosol, Mitochondrion, nucleus, cytoplasm, protein-containing complex.
• 数据链接 SwissProt: P55211 Human
  SwissProt: Q8C3Q9 Mouse
  
• 其它名称
  • Caspase9
  • APAF-3 antibody
  • APAF3 antibody
  • Apoptosis related cysteine peptidase antibody
  • Apoptotic protease Mch-6 antibody
  • Apoptotic protease-activating factor 3 antibody
  • CASP-9 antibody
  • CASP9 antibody
  • CASP9_HUMAN antibody
  • Caspase 9 apoptosis related cysteine peptidase antibody
  • Caspase 9 Dominant Negative antibody
  • Caspase 9c antibody
  • Caspase-9 antibody
  • Caspase-9 subunit p10 antibody
  • ICE LAP6 antibody
  • ICE like apoptotic protease 6 antibody
  • ICE-LAP6 antibody
  • ICE-like apoptotic protease 6 antibody
  • MCH6 antibody
  • PPP1R56 antibody
  • protein phosphatase 1, regulatory subunit 56 antibody
  • RNCASP9 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IP: Use at an assay dependent concentration.

• 

  Fig1: Western blot analysis of Caspase-9 on different lysates with Rabbit anti-Caspase-9 antibody (ET1610-95) at 1/1,000 dilution.
  
  Lane 1: C2C12 cell lysate
  Lane 2: Hela cell lysate
  Lane 3: Jurkat cell lysate
  
  Lysates/proteins at 10 µg/Lane.
  
  Predicted band size: 46 kDa
  Observed band size: 46 kDa
  
  Exposure time: 2 minutes;
  
  12% SDS-PAGE gel.
  
  Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-95) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Western blot analysis of Caspase-9 on different lysates with Rabbit anti-Caspase-9 antibody (ET1610-95) at 1/1,000 dilution.
  
  Lane 1: Hela-si NT cell lysate
  Lane 2: Hela-si Caspase-9 cell lysate
  
  Lysates/proteins at 10 µg/Lane.
  
  Predicted band size: 46 kDa
  Observed band size: 46 kDa
  
  Exposure time: 2 minutes;
  
  4-20% SDS-PAGE gel.
  
  ET1610-95 was shown to specifically react with Caspase-9 in Hela-si NT cells. Weakened band was observed when Hela-si Caspase-9 sample was tested. Hela-si NT and Hela-si Caspase-9 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-95, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

• 背景文献

• 1. Arango-Gonzalez B et al. Identification of a common non-apoptotic cell death mechanism in hereditary retinal degeneration. PLoS One 9:e112142 (2014).
  
  2. Schattenberg JM et al. Increased hepatic fibrosis and JNK2-dependent liver injury in mice exhibiting hepatocyte-specific deletion of cFLIP. Am J Physiol Gastrointest Liver Physiol 303:G498-506 (2012).

• Publishing research using Anti-Caspase-9 Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  36706851 Multifunctional gold nanorods in low-temperature photothermal interactions for combined tumor starvation and RNA interference therapy Author: Fan, R., Chen, C., Hu, J., Mu, M., Chuan, D., Chen, Z., Guo, G., & Xu, J. Published: 2023 Application: WB Species: Mouse Journal: Acta Biomaterialia IF: 10.633

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.