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概述
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产品描述
Caspase-9 belongs to a family of caspases, cysteine-aspartic proteases involved in apoptosis and cytokine signalling. Apoptotic signals cause the release of cytochrome c from mitochondria and activation of apaf-1 (apoptosome), which then cleaves the pro-enzyme of caspase-9 into the active dimer form. Regulation of this enzyme occurs through phosphorylation by an allosteric inhibitor, inhibiting dimerization and inducing a conformational change. Correct caspase-9 function is required for apoptosis, leading to the normal development of the central nervous system. Caspase-9 has multiple additional cellular functions that are independent of its role in apoptosis. Nonapoptotic roles of caspase-9 include regulation of necroptosis, cellular differentiation, innate immune response, sensory neuron maturation, mitochondrial homeostasis, corticospinal circuit organization, and ischemic vascular injury.
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产品名称
Anti-Caspase-9 Recombinant Rabbit Monoclonal Antibody
[SC65-05]
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分子量
Predicted band size: 46/30/17/37 kDa
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种属反应性
Human,Mouse
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验证应用
WB,IP
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抗体类型
重组兔单抗
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免疫原
Recombinant protein within Human Caspase-9 aa 95-335 / 416.
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偶联
Non-conjugated
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RRID
AB_2940743
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性能
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形态
Liquid
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浓度
1 mg/mL.
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存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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亚型
IgG
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纯化方式
Protein A affinity purified.
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亚细胞定位
Apoptosome, cytosol, Mitochondrion, nucleus, cytoplasm, protein-containing complex.
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数据链接
SwissProt: P55211 Human
SwissProt: Q8C3Q9 Mouse
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其它名称
- Caspase9
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
- Apoptotic protease Mch-6 antibody
- Apoptotic protease-activating factor 3 antibody
- CASP-9 antibody
- CASP9 antibody
- CASP9_HUMAN antibody
- Caspase 9 apoptosis related cysteine peptidase antibody
- Caspase 9 Dominant Negative antibody
- Caspase 9c antibody
- Caspase-9 antibody
- Caspase-9 subunit p10 antibody
- ICE LAP6 antibody
- ICE like apoptotic protease 6 antibody
- ICE-LAP6 antibody
- ICE-like apoptotic protease 6 antibody
- MCH6 antibody
- PPP1R56 antibody
- protein phosphatase 1, regulatory subunit 56 antibody
- RNCASP9 antibody
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应用
WB: 1:500-1:2,000
IP: Use at an assay dependent concentration.
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Fig1: Western blot analysis of Caspase-9 on different lysates with Rabbit anti-Caspase-9 antibody (ET1610-95) at 1/1,000 dilution.
Lane 1: C2C12 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-95) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of Caspase-9 on different lysates with Rabbit anti-Caspase-9 antibody (ET1610-95) at 1/1,000 dilution.
Lane 1: Hela-si NT cell lysate
Lane 2: Hela-si Caspase-9 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
ET1610-95 was shown to specifically react with Caspase-9 in Hela-si NT cells. Weakened band was observed when Hela-si Caspase-9 sample was tested. Hela-si NT and Hela-si Caspase-9 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-95, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
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背景文献
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1. Arango-Gonzalez B et al. Identification of a common non-apoptotic cell death mechanism in hereditary retinal degeneration. PLoS One 9:e112142 (2014).
2. Schattenberg JM et al. Increased hepatic fibrosis and JNK2-dependent liver injury in mice exhibiting hepatocyte-specific deletion of cFLIP. Am J Physiol Gastrointest Liver Physiol 303:G498-506 (2012).
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Publishing research
using Anti-Caspase-9 Recombinant Rabbit Monoclonal Antibody? Please let us know so
that we can cite the reference in this datasheet.
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PMID:
36706851
Multifunctional gold nanorods in low-temperature photothermal interactions for combined tumor starvation and RNA interference therapy
Author: Fan, R., Chen, C., Hu, J., Mu, M., Chuan, D., Chen, Z., Guo, G., & Xu, J. Published: 2023 Application: WB Species: Mouse Journal: Acta Biomaterialia IF: 10.633
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Specific Protocols
To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you
get on.
Click here to view the
general protocols