RRM2 Recombinant Rabbit Monoclonal Antibody [JM93-43]
Catalog# ET1705-62
RRM2 Recombinant Rabbit Monoclonal Antibody [JM93-43]
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
Human
-
Mouse
-
Rat
概述
产品名称
RRM2 Recombinant Rabbit Monoclonal Antibody [JM93-43]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human RRM2 aa 1-147 / 389.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IF-Tissue, IHC-P
分子量
Predicted band size: 45 kDa
阳性对照
HeLa cell lysate, HDLM-2 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HeLa, human tonsil tissue, human appendix tissue.
偶联
unconjugated
克隆号
JM93-43
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
-
IF-Cell
-
1:100
-
IF-Tissue
-
1:20-1:50
-
IHC-P
-
1:200-1:1,000
靶点
功能
Belongs to the ribonucleoside diphosphate reductase small chain family. Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.it have two regulatory sites: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2.
背景文献
1. Rasmussen RD et al. BRCA1-regulated RRM2 expression protects glioblastoma cells from endogenous replication stress and promotes tumorigenicity. Nat Commun 7:13398 (2016).
2. Su YF et al. The expression of ribonucleotide reductase M2 in the carcinogenesis of uterine cervix and its relationship with clinicopathological characteristics and prognosis of cancer patients. PLoS One 9:e91644 (2014).
序列相似性
Belongs to the ribonucleoside diphosphate reductase small chain family.
翻译后修饰
Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.
亚细胞定位
Cytoplasm, Nucleus.
别名
cb111 antibody
chunp6884 antibody
etID309896.20 antibody
R2 antibody
reductase M2 polypeptide variant 1 antibody
reductase M2 polypeptide variant 2 antibody
reductase M2 polypeptide variant 3a antibody
reductase M2 polypeptide variant 3b antibody
reductase M2 polypeptide variant 3c antibody
reductase M2 polypeptide variant 3d antibody
展开图片
-
Western blot analysis of RRM2 on different lysates with Rabbit anti-RRM2 antibody (ET1705-62) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HDLM-2 cell lysate
Lane 3: Mouse liver tissue lysate
Lane 4: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-62) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling RRM2 with Rabbit anti-RRM2 antibody (ET1705-62) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RRM2 antibody (ET1705-62) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-RRM2 antibody (ET1705-62) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-RRM2 antibody (ET1705-62) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-62) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"