• 概述

• 产品描述 Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.
• 产品名称 Anti-Active Caspase-3 Recombinant Rabbit Monoclonal Antibody [SR01-02]
• 分子量 Predicted band size: 17 kDa
• 种属反应性 Human, Pig
• 验证应用 WB,IF-Cell,IF-Tissue,IHC-P
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within Human Caspase-3 aa 28-67 / 277.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cytoplasm
• 数据链接 SwissProt: P42574 Human
  
• 其它名称
  • Caspase3
  • A830040C14Rik antibody
  • Apopain antibody
  • CASP 3 antibody
  • CASP-3 antibody
  • CASP3 antibody
  • CASP3_HUMAN antibody
  • Casp3a antibody
  • Caspase 3 antibody
  • Caspase 3, apoptosis-related cysteine peptidase antibody
  • Caspase 3, apoptosis-related cysteine protease antibody
  • Caspase 3, apoptosis-related cysteine protease a antibody
  • Caspase-3 subunit p12 antibody
  • Caspase3 antibody
  • CC3 antibody
  • CPP 32 antibody
  • CPP-32 antibody
  • CPP32 antibody
  • CPP32B antibody
  • Cysteine protease CPP32 antibody
  • EC 3.4.22.56 antibody
  • ICE3 antibody
  • LICE antibody
  • mldy antibody
  • OTTHUMP00000165052 antibody
  • OTTHUMP00000165053 antibody
  • OTTHUMP00000165054 antibody
  • PARP cleavage protease antibody
  • Procaspase3 antibody
  • Protein Yama antibody
  • SCA 1 antibody
  • SCA-1 antibody
  • SCA1 antibody
  • SREBP cleavage activity 1 antibody
  • Yama antibody
  • Yama protein antibody
  • ActiveCaspase-3
  • ActiveCaspase3
  more

• 应用

  WB: 1:1,000-1:2,000
  IF-Cell: 1:50-1:200
  IF-Tissue: 1:50-1:200
  IHC-P: 1:50-1:200

• 

  Fig1: Western blot analysis of Active Caspase-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-47, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Camptothecin (2 μM) treated Jurkat cell lysate
  Lane 2: Untreated Jurkat cell lysate

  

  Fig2: ICC staining of Active Caspase-3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining of Active Caspase-3 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Active Caspase-3 antibody (ET1602-47) at 1/100 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-47) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Active Caspase-3 antibody (ET1602-47) at 1/100 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1602-47) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• 背景文献

• 1. Xu TX et al. Hypoxia responsive miR-210 promotes cell survival and autophagy of endometriotic cells in hypoxia. Eur Rev Med Pharmacol Sci 20:399-406 (2016).
  
  2. Huang X et al. Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro. Mol Med Rep 13:613-22 (2016).

• Publishing research using Anti-Active Caspase-3 Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  35506256 Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota Author: Wang, C., Wei, S., Liu, B., Wang, F., Lu, Z., Jin, M., & Wang, Y. Published: 2022 Application: WB Species: Pig Journal: Gut Microbes IF: 10.245
• PMID:
  35957979 Vitamin D Receptor Genetic Polymorphisms Associate With a Decreased Susceptibility to Extremity Osteomyelitis Partly by Inhibiting Macrophage Apoptosis Through Inhibition of Excessive ROS Production via VDR-Bmi1 Signaling Author: Zhao, X. Q., Wan, H. Y., He, S. Y., Qin, H. J., Yu, B., & Jiang, N. Published: 2022 Application: WB Species: S.aureus Journal: Front Physiol. IF: 4.755
• PMID:
  36555782 Phellinus baumii Polyphenol: A Potential Therapeutic Candidate against Lung Cancer Cells Author: Liu, X., Cui, S., Dan, C., Li, W., Xie, H., Li, C., & Shi, L. Published: 2022 Application: WB Species: Human Journal: Int J Mol Sci. IF: 6.208
• PMID:
  36180828 miR-351 promotes atherosclerosis in diabetes by inhibiting the ITGB3/PIK3R1/Akt pathway and induces endothelial cell injury and lipid accumulation Author: Li, H., Song, D., Liu, Q., Li, L., Sun, X., Guo, J., Li, D., & Li, P. Published: 2022 Application: WB Species: Mouse Journal: Mol Med. IF: 6.376
• PMID:
  35538475 N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma Author: Chen, Y., Lu, Z., Qi, C., Yu, C., Li, Y., Huan, W., Wang, R., Luo, W., Shen, D., Ding, L., Ren, L., Xie, H., Xue, D., Wang, M., Ni, K., Xia, L., Qian, J., & Li, G. Published: 2022 Application: WB Species: Human Journal: Mol Cancer. IF: 41.444
• PMID:
  36185307 Phosphoproteomics reveals that cinobufotalin promotes intrahepatic cholangiocarcinoma cell apoptosis by activating the ATM/CHK2/p53 signaling pathway Author: Xia, Z., Li, M., Hu, M., Lin, Y., Atteh, L. L., Fu, W., Gao, L., Bai, M., Huang, C., Yue, P., Liu, Y., & Meng, W. Published: 2022 Application: WB Species: Human Journal: Front Oncol. IF: 5.738
• PMID:
  35842652 Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth Author: Hu, J., Cao, J., Jin, R., Zhang, B., Topatana, W., Juengpanich, S., Li, S., Chen, T., Lu, Z., Cai, X., & Chen, M. Published: 2022 Application: WB Species: Human Journal: Cell Commun Signal . IF: 7.525
• PMID:
  33495517 Bis-isatin derivatives: design, synthesis, and biological activity evaluation as potent dimeric DJ-1 inhibitors. Acta pharmacologica Sinica, 10.1038/s41401-020-00600-5. Advance online publication. Author: Chen, X. B., Zhu, H. Y., Bao, K., Jiang, L., Zhu, H., Ying, M. D., He, Q. J., Yang, B., Sheng, R., & Cao, J. Published: 2021 Journal: Acta Pharmacol Sin IF: 5.064
• PMID:
  33740524 Aqueous extract of Taxus chinensis var. mairei regulates the Hippo-YAP pathway and promotes apoptosis of non-small cell lung cancer via ATF3 in vivo and in vitro. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 138, 111506. Author: Zhang, G., Dai, S., Chen, Y., Wang, H., Chen, T., Shu, Q., Chen, S., Shou, L., & Cai, X. Published: 2021 Application: WB Species: Mouse,Human Journal: Biomed Pharmacother IF: 4.545
• PMID:
  31322175 Silencing RRM2 inhibits multiple myeloma by targeting the Wnt/β-catenin signaling pathway Author: Dr Xia Liu,Professor Jianchao Wang Published: 2019 Application: WB Species: multiple myeloma cell Journal: MOLECULAR MEDICINE REPORTS IF: 1.851
• PMID:
  31345225 SAE1 promotes human glioma progression through activating AKT SUMOylation-mediated signaling pathways. Author: Ziwei Liang Published: 2019 Application: WB Species: glioma cells Journal: Cell Communication and Signaling IF: 5.111

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.