PDIA2 Recombinant Rabbit Monoclonal Antibody [JF97-08]
Catalog# ET1702-70
PDIA2 Recombinant Rabbit Monoclonal Antibody [JF97-08]
-
WB
-
Human
-
Mouse
概述
产品名称
PDIA2 Recombinant Rabbit Monoclonal Antibody [JF97-08]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human PDIA2 aa 405-450.
种属反应性
Human, Mouse
验证应用
WB
分子量
Predicted band size: 57 kDa
阳性对照
HEK-293 cell lysate, NIH/3T3 cell lysate, Human liver tissue lysate.
偶联
unconjugated
克隆号
JF97-08
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
靶点
功能
Oxidoreductase-protein disulfide isomerase (PDI) is a homodimer consisting of subunits that catalyzes thiol-disulfide exchange, mediates folding of newly synthesized proteins and functions as a molecular chaperone. PDI localizes to the lumen of the endoplasmic reticulum (ER), where in conjunction with folding-helper proteins, such as immunoglobulin heavy chain binding protein (BiP), mediates tertiary and quaternary protein-processing. Cell surface PDI induces sulfhydryl-mediated conformational changes in integrin-mediated adhesion receptor-ligand interactions, thereby regulating integrin responses and cell adhesion. Additionally, PDI functions as a subunit of two more complex enzyme systems: the prolyl-4-hydroxylase and the triacylglycerol transfer proteins.
背景文献
1. Obser T et al. Identification and characterization of the elusive mutation causing the historical von Willebrand Disease type IIC Miami. J Thromb Haemost 14:1725-35 (2016).
2. Koumangoye R & Delpire E The Ste20 kinases SPAK and OSR1 travel between cells through exosomes. Am J Physiol Cell Physiol 311:C43-53 (2016).
序列相似性
Belongs to the protein disulfide isomerase family.
亚细胞定位
Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome, Cell membrane.
别名
Pancreas specific protein disulfide isomerase antibody
Pancreas-specific protein disulfide isomerase antibody
Pancreatic protein disulfide isomerase antibody
PDA2 antibody
PDI antibody
PDIA2 antibody
PDIA2_HUMAN antibody
PDIp antibody
PDIR antibody
Protein disulfide isomerase A2 antibody
展开图片
-
Western blot analysis of PDIA2 on different lysates with Rabbit anti-PDIA2 antibody (ET1702-70) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 92 kDa
Observed band size: 92 kDa
Exposure time: Lane 1: 1 minute 18 seconds; Lane 2-3: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-70) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"