TRIM29 Mouse Monoclonal Antibody [8C8G5]
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Catalog# EM1711-28
TRIM29 Mouse Monoclonal Antibody [8C8G5]
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WB
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IF-Cell
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FC
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Human
概述
产品名称
TRIM29 Mouse Monoclonal Antibody [8C8G5]
抗体类型
Mouse Monoclonal Antibody
免疫原
Purified recombinant fragment of human TRIM29 (AA: 451-588) expressed in E. Coli.
种属反应性
Human
验证应用
WB, IF-Cell, FC
分子量
65.8kDa
阳性对照
Hela, HepG2, LOVO, and A431 cell Hela cells HL-60 cells
偶联
unconjugated
克隆号
8C8G5
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG2a
纯化方式
Protein G affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IF-cell
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1:50-1:200
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FC
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1:100-1:200
靶点
功能
The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype.
背景文献
1. Oncotarget. 2016 Mar 22;7(12):13634-50.
2. Dis Markers. 2014;2014:317817.
亚细胞定位
Cytoplasm. Colocalizes with intermediate filaments.
UNIPROT
别名
Ataxia telangiectasia group D associated protein antibody
Ataxia telangiectasia group D-associated protein antibody
ATDC antibody
FLJ36085 antibody
TRI29_HUMAN antibody
TRIM 29 antibody
TRIM29 antibody
Tripartite motif containing 29 antibody
Tripartite motif containing protein 29 antibody
Tripartite motif protein 29 antibody
展开图片
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Western blot analysis of TRIM29 against human TRIM29 (AA: 451-588) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of EM1711-28 against HEK293 (1) and TRIM29 (AA: 451-588)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of EM1711-28 against Hela (1), HepG2 (2), LOVO (3), and A431 (4) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunocytochemistry staining of TRIM29 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1711-28, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue), Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
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Flow cytometric analysis of TRIM29 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-28, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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