• 概述

• 产品描述 The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes. Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.
• 产品名称 Anti-PARP Recombinant Rabbit Monoclonal Antibody [SU03-68]
• 分子量 Predicted band size: 113 kDa
• 种属反应性 Human,Mouse, Rat
• 验证应用 WB,IF-Cell,IF-Tissue,IHC-P,FC
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within N-terminal human PARP.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Nucleus, nucleolus, chromosome.
• 数据链接 SwissProt: P09874 Human
  SwissProt: P11103 Mouse
  SwissProt: P27008 Rat
  
• 其它名称
  • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
  • ADP ribosyltransferase diphtheria toxin like 1 antibody
  • ADP ribosyltransferase NAD(+) antibody
  • ADPRT 1 antibody
  • ADPRT antibody
  • ADPRT1 antibody
  • ARTD1 antibody
  • msPARP antibody
  • NAD(+) ADP ribosyltransferase 1 antibody
  • NAD(+) ADP-ribosyltransferase 1 antibody
  • pADPRT 1 antibody
  • pADPRT1 antibody
  • PARP 1 antibody
  • PARP antibody
  • PARP-1 antibody
  • PARP1 antibody
  • PARP1_HUMAN antibody
  • Poly (ADP ribose) polymerase 1 antibody
  • poly (ADP ribose) polymerase family, member 1 antibody
  • Poly [ADP-ribose] polymerase 1 antibody
  • Poly(ADP ribose) polymerase antibody
  • poly(ADP ribose) synthetase antibody
  • poly(ADP ribosyl)transferase antibody
  • Poly[ADP ribose] synthetase 1 antibody
  • Poly[ADP-ribose] synthase 1 antibody
  • PPOL antibody
  more

• 应用

  WB: 1:2,000
  IF-Cell: 1:500
  IF-Tissue: 1:50-1:200
  IHC-P: 1:500
  FC: 1:1,000

• 

  Fig1: Western blot analysis of PARP on different lysates with Rabbit anti-PARP antibody (ET1608-56) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
  
  Lane 1: HeLa cell lysate (15 µg/Lane)
  Lane 2: Jurkat cell lysate (15 µg/Lane)
  Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
  Lane 4: C2C12 cell lysate (15 µg/Lane)
  Lane 5: C6 cell lysate (15 µg/Lane)
  Lane 6: PC-12 cell lysate (15 µg/Lane)
  
  Predicted band size: 113 kDa
  Observed band size: 113 kDa
  
  Exposure time: 6 seconds;
  
  4-20% SDS-PAGE gel.
  
  Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-56) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Western blot analysis of PARP on different lysates with Rabbit anti-PARP antibody (ET1608-56) at 1/2,000 dilution.
  
  Lane 1: HeLa whole cell lysate
  Lane 2: HeLa treated with 1μM staurosporine for 3 hours whole cell lysate
  
  Lysates/proteins at 20 µg/Lane.
  
  Predicted band size: 113 kDa
  Observed band size: 113/28 kDa
  
  Exposure time: 1 minute 9 seconds;
  
  4-20% SDS-PAGE gel.
  
  Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-56) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  

  Fig3: All lanes: Western blot analysis of PARP with anti-PARP antibody[SU03-68] (ET1608-56) at 1:500 dilution.
  Lane 1/2: Wild-type Hela whole cell lysate (10 µg).
  Lane 3/4: PARP knockdown Hela whole cell lysate (10 µg).
  
  ET1608-56 was shown to specifically react with PARP in wild-type Hela cells. Weakened bands were observed when PARP knockdown samples were tested. Wild-type and PARP knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1608-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  

  Fig4: Immunocytochemistry analysis of HeLa cells labeling PARP with Rabbit anti-PARP antibody (ET1608-56) at 1/500 dilution.
  
  Cells were fixed in 100% methanol for 5 minutes, blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PARP antibody (ET1608-56) at 1/500 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PARP antibody (ET1608-56) at 1/500 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1608-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-PARP antibody (ET1608-56) at 1/500 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1608-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig7: Flow cytometric analysis of HeLa cells labeling PARP.
  
  Cells were fixed and permeabilized. Then stained with the primary antibody (ET1608-56, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  

  Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-PARP antibody (ET1608-56) at 1/500 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1608-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• 背景文献

• 1. Cao C et al. The long intergenic noncoding RNA UFC1, a target of MicroRNA 34a, interacts with the mRNA stabilizing protein HuR to increase levels of -catenin in HCC cells. Gastroenterology 148:415-26.e18 (2015).
  
  2. Gao S et al. Ischemia-reperfusion injury of the retina is linked to necroptosis via the ERK1/2-RIP3 pathway. Mol Vis 20:1374-87 (2014).

• Publishing research using Anti-PARP Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  36555782 Phellinus baumii Polyphenol: A Potential Therapeutic Candidate against Lung Cancer Cells Author: Liu, X., Cui, S., Dan, C., Li, W., Xie, H., Li, C., & Shi, L. Published: 2022 Application: WB Species: Human Journal: Int J Mol Sci. IF: 6.208
• PMID:
  36198708 BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency Author: Wang, F., Liu, Y., Ni, F., Jin, J., Wu, Y., Huang, Y., Ye, X., Shen, X., Ying, Y., Chen, J., Chen, R., Zhang, Y., Sun, X., Wang, S., Xu, X., Chen, C., Guo, J., & Zhang, D. Published: 2022 Application: WB Species: Mouse Journal: Nat Commun. IF: 17.694
• PMID:
  35538475 N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma Author: Chen, Y., Lu, Z., Qi, C., Yu, C., Li, Y., Huan, W., Wang, R., Luo, W., Shen, D., Ding, L., Ren, L., Xie, H., Xue, D., Wang, M., Ni, K., Xia, L., Qian, J., & Li, G. Published: 2022 Application: WB Species: Human Journal: Mol Cancer. IF: 41.444
• PMID:
  35842652 Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth Author: Hu, J., Cao, J., Jin, R., Zhang, B., Topatana, W., Juengpanich, S., Li, S., Chen, T., Lu, Z., Cai, X., & Chen, M. Published: 2022 Application: WB Species: Human Journal: Cell Commun Signal . IF: 7.525
• PMID:
  33609560 Fangchinoline exerts anticancer effects on colorectal cancer by inducing autophagy via regulation AMPK/mTOR/ULK1 pathway. Biochemical pharmacology, 186, 114475. Author: Xiang, X., Tian, Y., Hu, J., Xiong, R., Bautista, M., Deng, L., Yue, Q., Li, Y., Kuang, W., Li, J., Liu, K., Yu, C., & Feng, G. Published: 2021 Application: WB Species: Human Journal: Biochem Pharmacol IF: 4.96
• PMID:
  32597573 Exopolysaccharide from Cryptococcus heimaeyensis S20 induces autophagic cell death in non-small cell lung cancer cells via ROS/p38 and ROS/ERK signalling Author: Chao Shen Published: 2020 Application: WB,IHC Species: human Journal: Cell Prolif. IF: 5.753
• PMID:
  31322175 Silencing RRM2 inhibits multiple myeloma by targeting the Wnt/β-catenin signaling pathway Author: Dr Xia Liu,Professor Jianchao Wang Published: 2019 Application: WB Species: multiple myeloma cell Journal: MOLECULAR MEDICINE REPORTS IF: 1.851

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.