KIBRA Rabbit Polyclonal Antibody
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Catalog# ER1911-87
KIBRA Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
概述
产品名称
KIBRA Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human KIBRA 351-450/1113
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue
分子量
125 KDa
阳性对照
Expressed in mammary epithelial cells and breast cancer cell lines. Found in the luminal epithelium surrounding the ducts in the normal breast. In the brain, expressed in somatodendritic compartment of neurons in the cortex and hippocampus and in the cerebellum it is found in the Purkinje cells and some granule cells (at protein level). Detected in brain, heart, colon and kidney. In the kidney, expressed in glomerular podocytes, in some tubules and in the collecting duct.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2000
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IHC-P
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1:100-500
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IF-tissue
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1:100-500
靶点
功能
KIBRA is a WW domain containing protein, first identified by yeast 2-hybrid analysis of a human brain cDNA expression library. Gene expression studies have shown that KIBRA is expressed in memory-related brain structures including the hippocampus (dentate gyrus and the CA1 region) and the temporal lobe, 2 key regions for memory, and that the expression of specific KIBRA alleles is significantly associated with memory performance.
背景文献
暂无
亚细胞定位
Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Cell projection, ruffle membrane. Note=Co-localizes with PRKCZ in the perinuclear region.
UNIPROT
别名
FLJ10865 antibody
FLJ23369 antibody
HBeAg-binding protein 3 antibody
HBEBP3 antibody
HBEBP36 antibody
KIAA0869 antibody
KIBRA antibody
Kidney and brain protein antibody
Protein KIBRA antibody
Protein WWC1 antibody
展开图片
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Sample:
Siha(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
U-2OS(Human) Cell Lysate at 30 ug
Primary: Anti- KIBRA (ER1911-87) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kD
Observed band size:125 kD -
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (ER1911-87) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (ER1911-87) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (ER1911-87) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (ER1911-87) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (ER1911-87) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (ER1911-87) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (ER1911-87) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIBRA) Polyclonal Antibody, Unconjugated (ER1911-87) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"