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Rabbit Anti-Mouse IgG polyclonal Antibody

RMB: 200

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Catalog# HA1030

Rabbit Anti-Mouse IgG polyclonal Antibody

Application

  • WB

  • ELISA

  • IP

Reactivity

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Rabbit Anti-Mouse IgG polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Mouse IgG

种属反应性

Mouse

验证应用

WB, ELISA, IP

阳性对照

Mouse IgG.

偶联

unconjugated

产品特性

形态

Liquid

浓度

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4).

亚型

IgG

纯化方式

Mouse IgG affinity purified.

应用稀释度

  • WB

  • 1:1000-1:5000

  • ELISA

  • 1:1000-1:5000

  • IP

  • 1:2000

靶点

功能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

背景文献

暂无

图片

  • Western blot analysis of Rabbit Anti-Mouse IgG on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA1030" style="font-weight: bold;text-decoration: underline;">HA1030</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Mouse IgG lysate<br />Lane 2: Human IgG lysate

    Western blot analysis of Rabbit Anti-Mouse IgG on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA1030, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse IgG lysate
    Lane 2: Human IgG lysate

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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