Anti-MARCH9 antibody
ER1903-04
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep
Applications: WB, FC
Molecular Wt: 38 KDa
Description:
E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Immunogen:
KLH conjugated synthetic peptide derived from human MARCH9 201-300/346
Positive control:
Ubiquitously expressed.
Subcellular location:
Golgi apparatus membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.
Database links:
SwissProt: Q86YJ5 Human
Recommended Dilutions:
  

WB1:500-2000
FC1μg/Test
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Instruction:
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Purity:
affinity purified by Protein A
Images
ER1903-04_1_small.jpg
Fig1: Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).
ER1903-04_2_small.jpg
Fig2: Sample: MCF-7 (human)cell Lysate at 40 ug
Primary: Anti- MARCH9(ER1903-04)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38 kD
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.