Anti-KDM2A antibody [5C11F7]
EM1712-20
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB
Molecular Wt: 132.8kDa
Clone number: 5C11F7
Description:
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants.
Immunogen:
Purified recombinant fragment of human KDM2A (AA: 1-150) expressed in E. Coli.
Positive control:
Widely expressed, with highest levels in brain, testis and ovary, followed by lung.
Subcellular location:
Nucleus.
Database links:
SwissProt: Q9Y2K7 Human
Recommended Dilutions:
  

WB 1:500-1:2,000
Storage Buffer:
1*PBS with 0.05% sodium azide.
Storage Instruction:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity:
Protein G purified.
Images
EM1712-20_1_small.jpg
Fig1: Western blot analysis of KDM2A against human KDM2A (AA: 1-150) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
EM1712-20_2_small.jpg
Fig2: Western blot analysis of KDM2A against HEK293 (1) and KDM2A (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.
Background References
1.
Mol Cancer Res. 2018 Jan;16(1):58-68.
2.
Cancer Biomark. 2017 Jul 19;20(1):23-29.