• 概述

• 产品描述 Tubulin in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Tubulin-binding drugs kill cancerous cells by inhibiting microtubule dynamics, which are required for DNA segregation and therefore cell division. In eukaryotes, there are six members of the tubulin superfamily, although not all are present in all species. Both α and β tubulins have a mass of around 50 kDa and are thus in a similar range compared to actin (with a mass of ~42 kDa). In contrast, tubulin polymers (microtubules) tend to be much bigger than actin filaments due to their cylindrical nature. Tubulin was long thought to be specific to eukaryotes. More recently, however, several prokaryotic proteins have been shown to be related to tubulin.
• 产品名称 Anti-Beta tubulin Mouse Monoclonal Antibody [1-B11]
• 分子量 Predicted band size: 50 kDa
• 种属反应性 Human,Mouse,Rat,Zebrafish
• 验证应用 WB,IF-Cell,IHC-P,FC
• 抗体类型 小鼠单抗
• 免疫原 Synthetic peptide within human Beta tubulin aa 51-100.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 2 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG1
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cytoplasm ,cytoskeleton
• 数据链接 SwissProt: P07437 Human
  SwissProt: P99024 Mouse
  SwissProt: P69897 Rat
  
• 其它名称
  • Beta 4 tubulin antibody
  • Beta 5 tubulin antibody
  • BetaTubulin antibody
  • TBB5_HUMAN antibody
  • TUBB antibody
  • TUBB2 antibody
  • TUBB2A antibody
  • TUBB5 antibody
  • tubulin beta 2A antibody
  • Tubulin beta chain antibody
  • Tubulin beta-5 chain antibody
  more

• 应用

  WB: 1:5,000-1:20,000
  IF-Cell: 1:1,000
  IHC-P: 1:20
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of Beta tubulin on different lysates with Mouse anti-Beta tubulin antibody (EM0103) at 1/20,000 dilution.
  
  Lane 1: K-562 cell lysate
  Lane 2: Jurkat cell lysate
  Lane 3: HeLa cell lysate
  Lane 4: NIH/3T3 cell lysate
  Lane 5: RAW264.7 cell lysate
  Lane 6: C6 cell lysate
  
  Lysates/proteins at 15 µg/Lane.
  
  Predicted band size: 50 kDa
  Observed band size: 50 kDa
  
  Exposure time: 1 minute 40 seconds;
  
  4-20% SDS-PAGE gel.
  
  Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0103) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:50,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Immunocytochemistry analysis of Hela cells labeling Beta tubulin with Mouse anti-Beta tubulin antibody (EM0103) at 1/1,000 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta tubulin antibody (EM0103) at 1/1,000 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  

  Fig3: Immunocytochemistry analysis of HepG2 cells labeling Beta tubulin with Mouse anti-Beta tubulin antibody (EM0103) at 1/1,000 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta tubulin antibody (EM0103) at 1/1,000 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  

  Fig4: Immunocytochemistry analysis of NIH/3T3 cells labeling Beta tubulin with Mouse anti-Beta tubulin antibody (EM0103) at 1/500 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta tubulin antibody (EM0103) at 1/500 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin.

  

  Fig6: Flow cytometric analysis of Hela cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

  

  Fig7: Western blot analysis of β-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-β-tubulin antibody at 1/500 dilution.

• 背景文献

• 1. "Evolutionary history of a multigene family: an expressed human beta-tubulin gene and three processed pseudogenes." Lee M.G.-S., Lewis S.A., Wilde C.D., Cowan N.J. Cell 33:477-487(1982)
  
  2. "Tubulins in the primate retina: evidence that xanthophylls may be endogenous ligands for the paclitaxel-binding site." Crabtree D.V., Ojima I., Geng X., Adler A.J. Bioorg. Med. Chem. 9:1967-1976(2000)
  
  3. "Mutations in the beta-tubulin gene TUBB5 cause microcephaly with structural brain abnormalities." Breuss M., Heng J.I., Poirier K., Tian G., Jaglin X.H., Qu Z., Braun A., Gstrein T., Ngo L., Haas M., Bahi-Buisson N., Moutard M.L., Passemard S., Verloes A., Gressens P., Xie Y., Robson K.J., Rani D.S. Cell Rep. 2:1554-1562(2011)

• Publishing research using Anti-Beta tubulin Mouse Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  35110683 Ubiquitination of NLRP3 by gp78/Insig-1 restrains NLRP3 inflammasome activation Author: Xu, T., Yu, W., Fang, H., Wang, Z., Chi, Z., Guo, X., Jiang, D., Zhang, K., Chen, S., Li, M., Guo, Y., Zhang, J., Yang, D., Yu, Q., Wang, D., & Zhang, X. Published: 2022 Application: WB Species: Mouse Journal: Cell Death & Differentiation IF: 15.828
• PMID:
  36088805 LACTB suppresses migration and invasion of glioblastoma via downregulating RHOC/Cofilin signaling pathway Author: Hu, Y., Liu, H., Zhu, Z., Qi, X., Yuan, W., Tian, M., Wang, D., & Xu, J. Published: 2022 Application: WB Species: Human Journal: Biochem Biophys Res Commun. IF: 3.322
• PMID:
  33490072 Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis. Frontiers in cell and developmental biology, 8, 609090. Author: Lv, J. J., Wang, H., Cui, H. Y., Liu, Z. K., Zhang, R. Y., Lu, M., Li, C., Yong, Y. L., Liu, M., Zhang, H., Zhang, T. J., Zhang, K., Li, G., Nan, G., Zhang, C., Guo, S. P., Wang, L., Chen, Z. N., & Bian, H. Published: 2021 Application: WB Species: Mouse Journal: Front Cell Dev Biol IF: 5.201
• PMID:
  34930213 Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody Author: Zhao, J., Xu, J., Yang, T., Yu, X., Cheng, C., Zhang, T., Ren, Z., Li, N., Yang, F., & Li, G. Published: 2021 Application: WB Species: Human Journal: BMC Biotechnol. IF: 3.329
• PMID:
  34023714 Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish. Food chemistry, 360, 129814. Author: Zhang, D. G., Zhao, T., Hogstrand, C., Ye, H. M., Xu, X. J., & Luo, Z. Published: 2021 Application: WB Species: Yellow catfish Journal: Food Chem IF: 7.514
• PMID:
  32266878 Bromodomains and Extra-Terminal (BET) Inhibitor JQ1 Suppresses Proliferation of Acute Lymphocytic Leukemia by Inhibiting c-MycMediated Glycolysis Author: Cai-Wen Duan;Bin-Bing S. Zhou Published: 2020 Application: WB Species: human Journal: Med Sci Monit. IF: None
• PMID:
  32937100 Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation Author: Di Wang Published: 2020 Application: WB,IP Species: mice Journal: Molecular Cell IF: 15.584
• PMID:
  30986512 Regular Swimming Exercise Attenuated Neuroma Pain in Rats: Involvement of Leptin and Adiponectin. Author: Dongping Du Published: 2019 Application: WB Species: rat Journal: The Journal of Pain IF: 5.424
• PMID:
  30366764 Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages Author: 王迪 Published: 2018 Application: WB Species: None Journal: Immunity. IF: 19.734
• PMID:
  29434774 Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37,Cx40 and Cx43 Author: Wen Wang,Lin-Tao Jia Published: 2018 Application: WB Species: rat Journal: Exp Ther Med IF: 1.261
• PMID:
  25378581 KIF5B transports BNIP-2 to regulate p38 mitogen-activated protein kinase activation and myoblast differentiation Author: Yi Ting Zhou,Boon Chuan Low Published: 2015 Application: WB Species: mouse Journal: Mol Biol Cell IF: 4.8

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.