TGF-β Fibrosis Pathway Antibody Kit
概述
试剂盒组分
产品包括 | 规格 | 应用 | 反应性 | MW(kDa) |
---|---|---|---|---|
alpha smooth muscle Actin[ET1607-53] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P,FC,mIHC | Human,Mouse,Rat | Predicted band size: 42 kDa |
COL1A1[ET1609-68] | 20µl | WB,IHC-P | Human,Cow | Predicted band size: 139 kDa |
Smad2/3[RT1566] | 20µl | WB,IP,IF,IHC-P | Human,Mouse,Rat | 55-60kDa |
Smad2[ET1604-22] | 20µl | WB,IF-Cell,IHC-P,IP,FC | Human,Mouse,Rat | Predicted band size: 52 kDa |
Phospho-Smad3(S423/S425)[ET1609-41] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P | Human,Mouse | Predicted band size: 48 kDa |
YKL-40 / CHI3L1[EM1902-14] | 20µl | WB,IHC-P,IF-Cell | Human,Mouse | 43 kDa |
TGF beta Receptor II[ER1917-66] | 20µl | WB,ELISA,IHC-P,IF | Human,Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit,Sheep | 62 KDa |
TGF beta 1[HA721143] | 20µl | WB,IHC-P | Human,Mouse,Rat | Predicted band size: 44 kDa |
HRP Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH[HA1031] | 100µl | IP,ELISA,IHC-P,WB | Rabbit | |
HRP Conjugated Goat anti-Mouse IgG[HA1006] | 100µl | WB,ELISA,IHC-P | Mouse |
产品描述
Transforming growth factor- β The fibrosis pathway Antibody Sampler Kit provides an economical method to study the activation of transforming growth factor- β/ Smad2 / 3 signaling pathway leads to the expression of Pro fibrogenic genes in cells or tissues, including alpha smooth muscle Actin, COL1A1/Collagen Ⅰ and YKL-40 / CHI3L1were up-regulated in activated fibroblasts. The kit contains enough antibodies to perform at least two Western blotting tests on each primary antibody.And also includes secondary reagent for detection of these antibodies.
产品特性
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
背景
Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis.</br> In the context of fibrosis, TGF-β signaling to SMAD2/3 is one of the biggest drivers of the profibrotic program.TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII).Activated type I receptors associate with SMAD2/3 and phosphorylate them on a conserved carboxy terminal SSXS motif.In the context of fibrosis, SMAD2/3 activation upregulates expression of profibrotic genes such as COL1A1 and other ECM modulators that modify the extracellular matrix of the tissue. TGF-β/ SMAD2/3 signaling also induces expression of α-Smooth Muscle Actin in fibroblasts, causing transformation of these cells to myofibroblasts. Injury to the tissue attracts macrophages and other immune cells and the fibrotic tissue soon becomes a site of inflammation. In this pro-fibrotic, pro-inflammatory environment, YKL-40, also known as Chitinase-3-like protein 1 (CHI3L1), is secreted. YKL-40 is a pro-inflammatory glycoprotein that also contributes to the progression of fibrosis.
数据链接
SwissProt: P62737 Mouse
SwissProt: P62738 Rat
SwissProt: P02452 Human
Entrez Gene: 282187 Cow
SwissProt: Q15796 Human
SwissProt: Q15796 Human
SwissProt: Q62432 Mouse
SwissProt: O70436 Rat
SwissProt: P84022 Human
SwissProt: Q8BUN5 Mouse
SwissProt: P36222 Human
SwissProt: Q61362 Mouse
SwissProt: P37173 Human
SwissProt: P01137 Human
SwissProt: P04202 Mouse
SwissProt: P17246 Rat
背景文献
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3. Derynck, R. et al. (2001) Nat Genet 29, 117-29.
4. Miyazono, K. et al. (2000) Adv Immunol 75, 115-57.
5. Meng, X.M. et al. (2016) Nat Rev Nephrol 12, 325-38.
6. Wu, G. et al. (2000) Science 287, 92-7.
7. Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
8. Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.
9. Bagalad, B.S. et al. J Oral Maxillofac Pathol 21, 462-3.
10. Mack, M. (2018) Matrix Biol 68-69, 106-21.
11. Johansen, J.S. (2006) Dan Med Bull 53, 172-209.
图片
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Western blot analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody (ET1607-53) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: L6 cell lysate
Lane 7: Mouse heart tissue lysate
Lane 8: Mouse skin tissue lysate
Lane 9: Rat heart tissue lysate
Lane 10: Rat skin tissue lysate
Lane 11: Rat smooth muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-53) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Smad2 on different lysates with Rabbit anti-Smad2 antibody (ET1604-22) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HT-29 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HL-60 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 58 kDa
Exposure time: 1 minute 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-22) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Phospho-Smad3(S423/S425) on different lysates with Rabbit anti-Phospho-Smad3(S423/S425) antibody (ET1609-41) at 1/2,000 dilution.
Lane 1: A549 whole cell lysate
Lane 2: A549 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate
Lane 3: C2C12 whole cell lysate
Lane 4: C2C12 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-41) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.