• 概述

• 产品描述 The Pax family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. Pax family genes are also involved in a variety of signal transduction pathways in the adult organism. Mutations in Pax proteins have been linked to disease and cancer in humans. For example, the human PAX5 gene encodes a B cell lineage-specific protein, Pax-5, also designated B cell specific activator protein or BSAP, which is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. Pax-5 functions to regulate not only B cell development, but also influences the balance between immunoglobulin secretion and B cell proliferation. Overexpression of Pax-5 has been implicated in cellular transformation, and in the case of small lymphocytic lymphomas with plasmacytoid differentiation, a t(9;14)(p13;q32) translocation resulting in the deregulation of PAX5 gene expression has been detected.
• 产品名称 Anti-PAX5 Recombinant Rabbit Monoclonal Antibody [JJ08-87]
• 分子量 Predicted band size: 42 kDa
• 种属反应性 Human, Mouse
• 验证应用 WB,IF-Cell,IF-Tissue,IHC-P,FC
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within human PAX5 aa 240-280.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Nucleus.
• 数据链接 SwissProt: Q02548 Human
  SwissProt: Q02650 Mouse
  
• 其它名称
  • B cell lineage specific activator antibody
  • B cell lineage specific activator protein antibody
  • B cell specific activator protein antibody
  • B cell specific transcription factor antibody
  • B-cell-specific transcription factor antibody
  • BSAP antibody
  • EBB-1 antibody
  • KLP antibody
  • Paired box 5 antibody
  • Paired box gene 5 (B cell lineage specific activator protein) antibody
  • Paired box gene 5 (B cell lineage specific activator) antibody
  • Paired box gene 5 antibody
  • Paired box homeotic gene 5 antibody
  • Paired box protein Pax 5 antibody
  • Paired box protein Pax-5 antibody
  • Paired domain gene 5 antibody
  • PAX 5 antibody
  • PAX5 antibody
  • PAX5_HUMAN antibody
  • Transcription factor PAX 5 antibody
  more

• 应用

  WB: 1:5,000
  IF-Cell: 1:50-1:200
  IF-Tissue: 1:50-1:200
  IHC-P: 1:400-1:1,000
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of PAX5 on different lysates with Rabbit anti-PAX5 antibody (ET1701-49) at 1/5,000 dilution.
  
  Lane 1: Ramos cell lysate
  Lane 2: Raji cell lysate
  Lane 3: Daudi cell lysate
  
  Lysates/proteins at 20 µg/Lane.
  
  Predicted band size: 42 kDa
  Observed band size: 50 kDa
  
  Exposure time: 3 minutes 10 seconds;
  
  4-20% SDS-PAGE gel.
  
  Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-49) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  

  Fig2: ICC staining of PAX5 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-49, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining of PAX5 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-49, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: ICC staining of PAX5 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-49, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-PAX5 antibody (ET1701-49) at 1/1,000 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1701-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-PAX5 antibody (ET1701-49) at 1/1,000 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1701-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PAX5 antibody (ET1701-49) at 1/400 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1701-49) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig8: Flow cytometric analysis of PAX5 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• 背景文献

• 1. Zhao L et al. Paired box 5 is a frequently methylated lung cancer tumour suppressor gene interfering -catenin signalling and GADD45G expression. J Cell Mol Med 20:842-54 (2016).
  
  2. Ren Y et al. Diagnostic utility of PAX2 and PAX5 in distinguishing non-small cell lung cancer from small cell lung cancer. Int J Clin Exp Pathol 8:14709-16 (2015).

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.